Gene Expression Data
Assay Details
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Assay
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Reference:
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J:67304
Bulchand S, et al., LIM-homeodomain gene Lhx2 regulates the formation of the cortical hem. Mech Dev. 2001 Feb;100(2):165-75
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2386926
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Gene symbol:
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Isl1
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Gene name:
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ISL1 transcription factor, LIM/homeodomain
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Probe:
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rat Isl1 cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
4g:
embryonic day 12.5
(more  )
Specimen
4g:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
Regionally restricted |
4g
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Expression in the ventral telencephalon.
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Specimen
4h:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Lhx2tm1Dra/Lhx2tm1Dra
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: medial ganglionic eminence |
Present |
Regionally restricted |
4h
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Authors report expression is remarkably similar to that in controls embryos, including fine details, such as the striped pattern of expression in the medial ganglionic eminence.
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Analysis Tools