Gene Expression Data
Assay Details
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Assay
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Reference:
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J:74631
Tian E, et al., Otx2 is required to respond to signals from anterior neural ridge for forebrain specification. Dev Biol. 2002 Feb 15;242(2):204-23
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2179007
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Gene symbol:
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En2
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Gene name:
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engrailed 2
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Probe:
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En2 cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
4K:
embryonic day 9.5
(more  )
Specimen
4K:
(close  )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4L:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Otx2tm1Sia/Otx2tm4Sia
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future brain |
Present |
Regionally restricted |
4L
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Authors report expression shifted anteriorly compared with wild type; not expressed in most anterior neurectoderm.
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Specimen
5I:
embryonic day 8.5
(more )
Specimen
5I:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5J:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Otx2tm1Sia/Otx2tm4Sia
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future brain |
Present |
Regionally restricted |
5J
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Authors report expression shifted anteriorly compared with wild type; not expressed in most anterior neurectoderm.
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Analysis Tools