Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:74243
Kawasaki T, et al., Requirement of neuropilin 1-mediated Sema3A signals in patterning of the sympathetic nervous system. Development. 2002 Feb;129(3):671-80
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:2176238
|
Gene symbol:
|
Nefm
|
Gene name:
|
neurofilament, medium polypeptide
|
|
Results
|
Specimen
1. G.:
(close )
Genetic Background:
|
involves: C57BL/6 * CBA * ICR
|
Mutant Allele(s):
|
Nrp1tm1Hfu/Nrp1tm1Hfu
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Nefm; red - Th (assay MGI:2176203).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Present |
Regionally restricted |
1G
|
Expression is detected in the spinal nerve fibers and sprouted cell processes along the nerves.
|
Specimen
4. C.:
embryonic day 9.25
(more )
Note: Embryo is at the 10-12 somite stage.
Specimen
4. C.:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.25
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Embryo is at the 10-12 somite stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: neural tube |
Present |
Regionally restricted |
4C
|
Expression is detected in nerve fibers near the neural tube.
|
Specimen
4. D.:
embryonic day 9.25; Nrp1tm1Hfu/ Nrp1tm1Hfu
(more )
Note: Embryo is at the 10-12 somite stage.
Specimen
4. D.:
(close )
Genetic Background:
|
involves: C57BL/6 * CBA * ICR
|
Mutant Allele(s):
|
Nrp1tm1Hfu/Nrp1tm1Hfu
|
Age:
|
embryonic day 9.25
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Embryo is at the 10-12 somite stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: neural tube |
Present |
Regionally restricted |
4D
|
Expression is detected in nerve fibers near the neural tube.
|
Specimen
not shown:
embryonic day 9.25; Nrp1tm1Hfu/Nrp1 +
(more )
Specimen
not shown:
(close )
Genetic Background:
|
involves: C57BL/6 * CBA * ICR
|
Mutant Allele(s):
|
Nrp1tm1Hfu/Nrp1+
|
Age:
|
embryonic day 9.25
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: neural tube |
Present |
Regionally restricted |
|
Expression is detected in nerve fibers near the neural tube.
|
|