Gene Expression Data
Assay Details
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Assay
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Reference:
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J:74243
Kawasaki T, et al., Requirement of neuropilin 1-mediated Sema3A signals in patterning of the sympathetic nervous system. Development. 2002 Feb;129(3):671-80
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2176203
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Gene symbol:
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Th
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Gene name:
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tyrosine hydroxylase
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Antibody:
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anti-TH
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
1A, 1D:
embryonic day 12.5
(more  )
Specimen
1A, 1D:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: dorsal aorta |
Present |
Regionally restricted |
1D
,
1A
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Expression is detected in sympathetic ganglia dorsolateral to the dorsal aorta.
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| TS20: sympathetic nervous system |
Present |
Regionally restricted |
1D
,
1A
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Expression is detected in sympathetic ganglia dorsolateral to the dorsal aorta.
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Specimen
1B:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA * ICR
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Mutant Allele(s):
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Nrp1tm1Hfu/Nrp1tm1Hfu
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: embryo |
Present |
Regionally restricted |
1B
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Expression is detected in widely distributed cells in regions medial to sympathetic ganglions, the upper arm, the abdominal wall. These cells are assumed to be sympathetic neurons.
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| TS20: upper arm |
Present |
Regionally restricted |
1B
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Expression is detected in widely distributed cells in regions medial to sympathetic ganglions, the upper arm, the abdominal wall. These cells are assumed to be sympathetic neurons.
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Specimen
1E:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA * ICR
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Mutant Allele(s):
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Nrp1tm1Hfu/Nrp1tm1Hfu
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1F:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA * ICR
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Mutant Allele(s):
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Nrp1tm1Hfu/Nrp1tm1Hfu
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: embryo |
Present |
Regionally restricted |
1F
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Authors report expression in the shoulder.
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Specimen
1C:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Sema3atm1.1Tyag/Sema3atm1.1Tyag
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: upper arm |
Present |
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1C
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Expression detected in the upper arm and axilla.
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Specimen
1G:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Sema3atm1.1Tyag/Sema3atm1.1Tyag
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Th; green - Nefm (assay MGI:2176238).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Regionally restricted |
1G
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Expression is detected in the spinal nerve fibers and sprouted cell processes along nerves.
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Specimen
1H:
embryonic day 12.5
(more )
Specimen
1H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1I:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA * ICR
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Mutant Allele(s):
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Nrp1tm1Hfu/Nrp1tm1Hfu
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Analysis Tools