Probe:	Msx1 cDNA2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS15: branchial arch	Present	Regionally restricted	1A/B	Expression in the mesenchyme of the branchial arches.
TS15: endocardial cushion	Present	Regionally restricted	1A/B	Expression in the components of the forming heart (e.g. endocardial cushions).
TS15: heart	Present	Regionally restricted	1A/B	Expression in the components of the forming heart (e.g. endocardial cushions).
TS15: forelimb bud ectoderm	Present	Regionally restricted	1A/B	Expression the mesenchyme and epithelium of the progress zones.
TS15: forelimb bud mesenchyme	Present	Regionally restricted	1A/B	Expression the mesenchyme and epithelium of the progress zones.
TS15: hindlimb ridge ectoderm	Present	Regionally restricted	1A/B	Expression the mesenchyme and epithelium of the progress zones.
TS15: hindlimb ridge mesenchyme	Present	Regionally restricted	1A/B	Expression the mesenchyme and epithelium of the progress zones.
TS15: caudal neuropore	Strong	Regionally restricted	1A/B	A band of expression in a narrow streak running from the most rostral areas of the forebrain to the tail neuropore along the line of neural tube closure.
TS15: future forebrain	Strong	Regionally restricted	1A/B	A band of expression in a narrow streak running from the most rostral areas of the forebrain to the tail neuropore along the line of neural tube closure.
TS15: future hindbrain	Strong	Regionally restricted	1A/B	A band of expression in a narrow streak running from the most rostral areas of the forebrain to the tail neuropore along the line of neural tube closure.
TS15: future midbrain	Strong	Regionally restricted	1A/B	A band of expression in a narrow streak running from the most rostral areas of the forebrain to the tail neuropore along the line of neural tube closure.
TS15: neural tube	Strong	Regionally restricted	1A/B	A band of expression in a narrow streak running from the most rostral areas of the forebrain to the tail neuropore along the line of neural tube closure.
TS15: Rathke's pouch	Absent		1A/B

Structure	Level	Pattern	Image	Note
TS19: 4th ventricle	Present	Regionally restricted	2A/B	Expression in the anlage of the posterior choroid plexus.
TS19: metencephalon roof	Present	Regionally restricted	2A/B	Expression in the anlage of the posterior choroid plexus.
TS19: telencephalon	Present	Regionally restricted	2A/B	Expression in the thinning neuroepithelium destined to become the lateral choroid plexus.
TS19: future adenohypophysis	Present	Regionally restricted	2A/B	Expression in the anterior pituitary.
TS19: skeleton	Present	Regionally restricted	2A/B	Expression in cranial precursors.
TS19: brain	Present	Regionally restricted	2A/B	Expression in meningal precursors and in the dorsal midline of the neural tube.
TS19: Rathke's pouch	Strong	Regionally restricted	2A/B	Expression in the epithelium of Rathke's pouch.

Structure	Level	Pattern	Image	Note
TS19: 4th ventricle	Present	Regionally restricted	3A/B	Expression in the anlage of the posterior choroid plexus.
TS19: metencephalon roof	Present	Regionally restricted	3A/B	Expression in the anlage of the posterior choroid plexus.
TS19: telencephalon	Present		3A/B	Expression in the thinning neuroepithelium destined to become the lateral choroid plexus.
TS19: skeleton	Present	Regionally restricted	3A/B	Expression in cranial precursors.
TS19: brain	Present	Regionally restricted	3A/B	Expression in meningal precursors.

Structure	Level	Pattern	Image	Note
TS20: choroid invagination	Present	Regionally restricted	4A/B	Expression in the thinning epithelium destined to become the lateral choroid plexus.

Structure	Level	Pattern	Image	Note
TS20: head mesenchyme	Present	Regionally restricted	5A/B	Expression in migrating mesenchyme adjacent to the invaginating choroid plexus.

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present	Regionally restricted	6A/B	Expression along the ventral aspect in the region of the forming tuberculum posterius which represents the posterior margin of the diencephalon and the area adjacent to the rostral end of the notochord.
TS20: nasal cavity epithelium	Present		6A/B
TS20: remnant of Rathke's pouch	Present		6A/B

Structure	Level	Pattern	Image	Note
TS20: remnant of Rathke's pouch	Present	Regionally restricted	8A/B	Expression is higher in the areas of Rathke's pouch most distal from the myencephalon.

Structure	Level	Pattern	Image	Note
TS20: remnant of Rathke's pouch	Present		8C
TS20: adenohypophysis pars anterior	Present		8C	The highest levels of expression are in the cellular outgrowths of proliferating cells that forms the pars distalis.

Structure	Level	Pattern	Image	Note
TS21: pinna mesenchyme condensation	Present		9A/B

Structure	Level	Pattern	Image	Note
TS21: pinna mesenchyme condensation	Present		9C/D

Structure	Level	Pattern	Image	Note
TS22: choroid plexus	Present		10A/B

Structure	Level	Pattern	Image	Note
TS22: choroid plexus	Present		10C/D

Structure	Level	Pattern	Image	Note
TS22: choroid plexus	Present		10E/F

Structure	Level	Pattern	Image	Note
TS22: choroid plexus	Present		10G/H

Structure	Level	Pattern	Image	Note
TS22: choroid plexus	Present		10I/J

Structure	Level	Pattern	Image	Note
TS23: chondrocranium	Present	Regionally restricted	11A/B	Expression in the perichodrium of the chondrocranium.
TS23: head mesenchyme	Present	Regionally restricted	11A/B	Expression in the mesenchyme derived from the second and third branchial arch which later froms the components of the middle ear and the styloid process.

Structure	Level	Pattern	Image	Note
TS17: Rathke's pouch	Present
TS17: brain	Present			Expression in the forming meninges.
TS17: skeleton	Present			Expression in the skull bone precursors.

Structure	Level	Pattern	Image	Note
TS20: cranium	Present			Expression in the skull bone precursor.
TS20: brain	Present			Expression in the forming meninges.

Structure	Level	Pattern	Image	Note
TS21: choroid plexus	Present
TS21: cranium	Present			Expression in the skull bone precursor.
TS21: brain	Present			Expression in the forming meninges.

Structure	Level	Pattern	Image	Note
TS22: cranium	Present			Expression in the skull bone precursor.
TS22: brain	Present			Expression in the forming meninges.

Structure	Level	Pattern	Image	Note
TS23: choroid plexus	Present
TS23: brain	Present			Expression in the forming meninges.