Gene Expression Data
Assay Details
Assay
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Reference:
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J:64588
Toresson H, et al., Genetic control of dorsal-ventral identity in the telencephalon: opposing roles for pax6 and gsh2. Development. 2000 Oct;127(20):4361-71
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2150332
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Gene symbol:
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Ascl1
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Gene name:
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achaete-scute family bHLH transcription factor 1
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Antibody:
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anti-MASH1
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Detection system:
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Secondary antibody
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Results
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Specimen
2B:
embryonic day 11.5
(more )
Specimen
2B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: telencephalon |
Present |
Regionally restricted |
2B
|
High levels in lateral ganglionic eminence (LGE) progenitors.
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Specimen
2H:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Gsx2tm1Ssp/Gsx2tm1Ssp
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Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: telencephalon |
Present |
Regionally restricted |
2H
|
Missing from a large portion of the LGE.
|
Specimen
3C:
embryonic day 12.5
(more )
Specimen
3C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: telencephalon |
Present |
Regionally restricted |
3C
|
Expressed in LGE ventricular zone (VZ).
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Specimen
3D:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Gsx2tm1Ssp/Gsx2tm1Ssp
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: telencephalon |
Present |
Regionally restricted |
3D
|
Authors report alterations in expression in the LGE; only in ventral half of LGE.
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Specimen
4A:
embryonic day 16.5
(more )
Specimen
4A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: telencephalon |
Present |
Regionally restricted |
4A
|
Boundary of expression corresponds to LGE/cortex angle.
|
Specimen
4B:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Gsx2tm1Ssp/Gsx2tm1Ssp
|
Age:
|
embryonic day 16.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: telencephalon |
Present |
Regionally restricted |
4B
|
Expression in progenitors throughout the LGE up to the LGE/cortex boundary.
|
Specimen
not shown:
embryonic day 16.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: telencephalon |
Present |
Regionally restricted |
|
Ectopic expression in progenitors.
|
Specimen
7C:
(close )
Genetic Background:
|
involves: 129S2/SvPas
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Mutant Allele(s):
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Gsx2tm1Ssp/Gsx2tm1Ssp Pax6Sey/Pax6Sey
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: telencephalon |
Present |
Regionally restricted |
7C
|
Expression throughout the LGE and into the ventrolateral cortex.
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