Gene Expression Data
Assay Details
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Assay
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Reference:
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J:64588
Toresson H, et al., Genetic control of dorsal-ventral identity in the telencephalon: opposing roles for pax6 and gsh2. Development. 2000 Oct;127(20):4361-71
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2150331
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Gene symbol:
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Pax6
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Gene name:
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paired box 6
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Antibody:
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anti-PAX6
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Detection system:
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Secondary antibody
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Results
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Specimen
1B:
embryonic day 10.5
(more  )
Specimen
1B:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: telencephalon |
Present |
Regionally restricted |
1B
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In ventral telencephalon, expressed in cortex; cells expressing high levels dorsal to domain of GSH2 expression in cortical ventricular zone (VZ).
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Specimen
1D:
embryonic day 12.5
(more )
Specimen
1D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
Regionally restricted |
1D
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High levels throughout cortical VZ and into lateral LGE. Low levels in LGE VZ. Graded along dorsal-ventral axis; highest in progenitor cells close to boundary. Also in a stream of cells emanating from boundary between cortical and striatal VZ.
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Specimen
1F:
embryonic day 16.5
(more )
Specimen
1F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: telencephalon |
Present |
Regionally restricted |
1F
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Expression marks boundary between the LGE and cortical VZ.
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Specimen
2D:
embryonic day 11.5
(more )
Specimen
2D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: telencephalon |
Present |
Regionally restricted |
2D
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Expressed in cortical progenitors.
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Specimen
2J:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Gsx2tm1Ssp/Gsx2tm1Ssp
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: telencephalon |
Present |
Regionally restricted |
2J
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Ectopic expression in lateral ganglionic eminence (LGE) progenitors.
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Specimen
3A:
embryonic day 12.5
(more )
Specimen
3A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
Regionally restricted |
3A
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High levels throughout cortical VZ and into lateral LGE. Low levels in LGE VZ. Graded along dorsal-ventral axis; highest in progenitor cells close to boundary. Also in a stream of cells emanating from boundary between cortical and striatal VZ.
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Specimen
3B:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Gsx2tm1Ssp/Gsx2tm1Ssp
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
Regionally restricted |
3B
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Authors report alterations in expression in the LGE; remains ectopically expressed in LGE occupying more than half of LGE VZ. Stream of Pax6-expressing cells in lateral LGE was severely reduced.
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Specimen
4A:
embryonic day 16.5
(more )
Specimen
4A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: telencephalon |
Present |
Regionally restricted |
4A
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Expression marks boundary between the LGE and cortical VZ.
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Specimen
4B:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Gsx2tm1Ssp/Gsx2tm1Ssp
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: telencephalon |
Present |
Regionally restricted |
4B
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Boundary of expression corresponds to LGE/cortex angle; not ectopically expressed in LGE progenitors.
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Analysis Tools