Probe:	Wnt5a probe1
Probe preparation:	Antisense RNA
Visualized with:	Alkaline phosphatase
Assay notes:	One- and two-color in situ hybridization was performed using digoxygenin- and/or fluorescein-labeled probes, detected with alkaline phosphatase-conjugated anti-digoxigenin or anti-fluorescein antibodies. Color reactions were carried out using different alkaline phosphatase substrates, resulting in different colored products as indicated in some of the figure legends.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	4D	The cortical hem (see below) is delineated by the expression of Wnt2b, Wnt3a and Wnt5a.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Strong	Regionally restricted	3H	Curved band of strong expression along medial face of telencephalon; marks the cortical hem.
TS20: head mesenchyme	Present	Regionally restricted	3H	Expression in head mesenchyme invading the choroid plexus epithelium to form the stroma.
TS20: choroid invagination	Absent		3H	No expression in the choroid plexus epithelium.

Structure	Level	Pattern	Image	Note
TS20: head mesenchyme	Present	Regionally restricted	3I	Expression in mesenchymal cells that are invading the medial wall of the telencephalon to form the stromal layer of the choroid plexus.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Weak		7B	In brains of non-exencephalic homozygous mutant mice, telencephalic expression severely downregulated (weak and diffuse). There is no expression in tissue that might correspond to the cortical hem.

Structure	Level	Pattern	Image	Note
TS20: inner ear	Strong			Strong expression at the otic vesicle and nasal epithelium.
TS20: nasal cavity epithelium	Strong			Strong expression at the otic vesicle and nasal epithelium.

Structure	Level	Pattern	Image	Note
TS21: cortical plate	Present	Regionally restricted	5C	Expression in the cortical plate of the entire medial cerebral cortex, including the hippocampus (postmitotic neurons in the pyramidal cell layer) and adjacent limbic cortical areas. Expression marks the shrinking cortical hem.
TS21: telencephalon	Present	Regionally restricted	5C	Expression in the cortical plate of the entire medial cerebral cortex, including the hippocampus (postmitotic neurons in the pyramidal cell layer) and adjacent limbic cortical areas. Expression marks the shrinking cortical hem.
TS21: head mesenchyme	Present		5C	Expression also labels head mesenchymal cells.

Structure	Level	Pattern	Image	Note
TS24: telencephalon	Present	Regionally restricted	5D	Overlapping expression of Wnt2b, Wnt3a and Wnt5a marks a few cells along the lateral margin of the hippocampal fimbria-fornix. Expression largely confined to the hippocampal dentate gyrus.

Structure	Level	Pattern	Image	Note
TS24: telencephalon	Absent			Five non-exencephalic homozygous mutants showed no expression in the medial telencephalon.