Probe:	Wnt3a probe4
Probe preparation:	Antisense RNA
Visualized with:	Alkaline phosphatase
Assay notes:	One- and two-color in situ hybridization was performed using digoxygenin- and/or fluorescein-labeled probes, detected with alkaline phosphatase-conjugated anti-digoxigenin or anti-fluorescein antibodies. Color reactions were carried out using different alkaline phosphatase substrates, resulting in different colored products as indicated in some of the figure legends.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Strong	Regionally restricted	4A	Strong expression in a medial band marking the cortical hem.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Strong	Regionally restricted	7K (left)	Expression in the cortical hem.
TS17: diencephalon	Present		7K (left)
TS17: future spinal cord	Present		7K (left)
TS17: hindbrain	Present		7K (left)

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Strong	Regionally restricted	4G	Strong expression in a medial band marking the cortical hem. Weaker expression in the choroid plaque at the telencephalic midline.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Weak	Regionally restricted	7K (right)	In non-exencephalic embryos, expression in the medial telencephalon was weak or barely detectable.
TS17: diencephalon	Present		7K (right)	Authors report relatively normal expression in diencephalon, hindbrain and spinal cord.
TS17: future spinal cord	Present		7K (right)	Authors report relatively normal expression in diencephalon, hindbrain and spinal cord.
TS17: hindbrain	Present		7K (right)	Authors report relatively normal expression in diencephalon, hindbrain and spinal cord.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted		Cortical hem is delineated by the epxression of Wnt2b, Wnt3a and Wnt5a.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Strong	Regionally restricted	3A	Authors state that Wnt3a shows the same characteristic curved band of expression along the medial face of the telencephalon as Wnt2b; marks the cortical hem.

Structure	Level	Pattern	Image	Note
TS20: choroid invagination	Absent			No expression in the choroid plexus epithelium.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Absent			Expression in the telencephalon was not detected, or weak and diffuse. No expression detected in tissue that might correspond to the cortical hem.
TS20: inner ear	Strong			Strong expression at the otic vesicle and nasal epithelium.
TS20: nasal cavity epithelium	Strong			Strong expression at the otic vesicle and nasal epithelium.
TS20: diencephalon	Present	Regionally restricted		In some mutant mice, expression at the dorsal midline of the diencephalon.
TS20: hindbrain	Present	Regionally restricted		Expression next to the fourth ventricle where Ttr-expressing choroid plexus formed.

Structure	Level	Pattern	Image	Note
TS23: telencephalon	Present	Regionally restricted		Overlapping expression of Wnt2b, Wnt3a and Wnt5a marks a few cells along the lateral margin of the hippocampal fimbria-fornix, the dorsal point of attachment of the choroid plexus epithelium to the neuroepithelium.

Structure	Level	Pattern	Image	Note
TS24: telencephalon	Present	Regionally restricted		Overlapping expression of Wnt2b, Wnt3a and Wnt5a marks a few cells along the lateral margin of the hippocampal fimbria-fornix, the dorsal point of attachment of the choroid plexus epithelium to the neuroepithelium.

Structure	Level	Pattern	Image	Note
TS24: telencephalon	Absent			Five non-exencephalic homozygous mutants showed no expression in the medial telencephalon.