Hand2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:59338 Clouthier DE, et al., Signaling pathways crucial for craniofacial development revealed by endothelin-A receptor-deficient mice. Dev Biol. 2000 Jan 1;217(1):10-24
Assay type:	RNA in situ
MGI Accession ID:	MGI:2135644
Gene symbol:	Hand2
Gene name:	heart and neural crest derivatives expressed 2

Probe:	dhand probe16
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

3A +/+ ©

Specimen 3A +/+: embryonic day 9.5 (more

)
Note: Theiler stage 16 was chosen to be consistent with other data from this manuscript.

Structure	Level	Pattern	Image	Note
TS16: 1st branchial arch	Present	Regionally restricted	3A +/+	Expression is in the distal 2/3 of the structure.
TS16: 2nd branchial arch	Present	Regionally restricted	3A +/+	Expression is in the distal 2/3 of the structure.

3B +/+ ©

Specimen 3B +/+: embryonic day 9.5 (more

)
Note: Theiler stage 16 was chosen to be consistent with other data from this manuscript.

Structure	Level	Pattern	Image	Note
TS16: 1st branchial arch	Present	Regionally restricted	3B +/+	Expression is in the distal 2/3 of the structure.
TS16: 2nd branchial arch	Present	Regionally restricted	3B +/+	Expression is in the distal 2/3 of the structure.

3A -/- ©

Specimen 3A -/-: embryonic day 9.5; Ednra^tm1Ywa/Ednra^tm1Ywa (more

)
Note: Theiler stage 16 was chosen to be consistent with other data from this manuscript.

Structure	Level	Pattern	Image	Note
TS16: 1st branchial arch	Absent		3A -/-
TS16: 2nd branchial arch	Absent		3A -/-

3B -/- ©

Specimen 3B -/-: embryonic day 9.5; Ednra^tm1Ywa/Ednra^tm1Ywa (more

)
Note: Theiler stage 16 was chosen to be consistent with other data from this manuscript.

Structure	Level	Pattern	Image	Note
TS16: 1st branchial arch	Absent		3B -/-
TS16: 2nd branchial arch	Absent		3B -/-

3E +/+ ©

Specimen 3E +/+: embryonic day 10.5 (more

)

Structure	Level	Image
TS17: 1st branchial arch mesenchyme	Present	3E +/+
TS17: 2nd branchial arch mesenchyme	Present	3E +/+
TS17: 3rd branchial arch mesenchyme	Present	3E +/+
TS17: 4th branchial arch mesenchyme	Present	3E +/+
TS17: 6th branchial arch mesenchyme	Present	3E +/+

3F +/+ ©

Specimen 3F +/+: embryonic day 10.5 (more

)

Structure	Level	Image
TS17: 1st branchial arch mesenchyme	Present	3F +/+
TS17: 2nd branchial arch mesenchyme	Present	3F +/+
TS17: 3rd branchial arch mesenchyme	Present	3F +/+
TS17: 4th branchial arch mesenchyme	Present	3F +/+
TS17: 6th branchial arch mesenchyme	Present	3F +/+

3E -/- ©

Specimen 3E -/-: embryonic day 10.5; Ednra^tm1Ywa/Ednra^tm1Ywa (more

)

Structure	Level	Pattern	Image	Note
TS17: branchial arch	Trace		3E -/-	Expression was almost completely absent.

3F -/- ©

Specimen 3F -/-: embryonic day 10.5; Ednra^tm1Ywa/Ednra^tm1Ywa (more

)

Structure	Level	Pattern	Image	Note
TS17: branchial arch	Trace		3F -/-	Expression was almost completely absent.

Specimen not shown: embryonic day 10.5; Ednra^tm1Ywa/Ednra^tm1Ywa (more

)

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mesenchyme	Trace	Regionally restricted		Expression is in the distal aspects of the arch.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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