Dlx2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:59338 Clouthier DE, et al., Signaling pathways crucial for craniofacial development revealed by endothelin-A receptor-deficient mice. Dev Biol. 2000 Jan 1;217(1):10-24
Assay type:	RNA in situ
MGI Accession ID:	MGI:2135638
Gene symbol:	Dlx2
Gene name:	distal-less homeobox 2

Probe:	Dlx2 cDNA10
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

1A +/+ ©

Specimen 1A +/+: embryonic day 9.0 (more

)

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mesenchyme derived from neural crest	Present		1A +/+	Expression in migrating crest cells extending from posterior midbrain/anterior hindbrain (rhombomeres2 and r4) into branchial arches 1 and 2.
TS15: 2nd branchial arch mesenchyme derived from neural crest	Present		1A +/+	Expression in migrating crest cells extending from posterior midbrain/anterior hindbrain (rhombomeres2 and r4) into branchial arches 1 and 2.

1A -/- ©

Specimen 1A -/-: embryonic day 9.0; Ednra^tm1Ywa/Ednra^tm1Ywa (more

)

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mesenchyme derived from neural crest	Present		1A -/-	Expression in migrating crest cells extending from posterior midbrain/anterior hindbrain (rhombomeres2 and r4) into branchial arches 1 and 2. Expression in this mutant is the same as in wild-type embryos.
TS15: 2nd branchial arch mesenchyme derived from neural crest	Present		1A -/-	Expression in migrating crest cells extending from posterior midbrain/anterior hindbrain (rhombomeres2 and r4) into branchial arches 1 and 2. Expression in this mutant is the same as in wild-type embryos.

2A +/+ ©

Specimen 2A +/+: embryonic day 9.5 (more

)
Note: Theiler stage 16 was chosen for this 9.5dpc embryo because of the existence of the 4th branchial arch.

Structure	Level	Image
TS16: 1st branchial arch ectoderm	Strong	2A +/+
TS16: 1st branchial arch mesenchyme	Strong	2A +/+
TS16: 2nd branchial arch	Strong	2A +/+
TS16: 3rd branchial arch	Strong	2A +/+
TS16: 4th branchial arch	Strong	2A +/+

2B +/+ ©

Specimen 2B +/+: embryonic day 9.5 (more

)
Note: Theiler stage 16 was chosen for this 9.5dpc embryo because of the existence of the 4th branchial arch.

Structure	Level	Image
TS16: 1st branchial arch ectoderm	Strong	2B +/+
TS16: 1st branchial arch mesenchyme	Strong	2B +/+
TS16: 2nd branchial arch	Strong	2B +/+
TS16: 3rd branchial arch	Strong	2B +/+
TS16: 4th branchial arch	Strong	2B +/+

2A -/- ©

Specimen 2A -/-: embryonic day 9.5; Ednra^tm1Ywa/Ednra^tm1Ywa (more

)
Note: Theiler stage 16 was chosen for this 9.5dpc embryo because of the existence of the 4th branchial arch.

Structure	Level	Image	Note
TS16: 1st branchial arch ectoderm	Strong	2A -/-	Expression in this mutant is the same as in wild-type embryos.
TS16: 1st branchial arch mesenchyme	Strong	2A -/-	Expression in this mutant is the same as in wild-type embryos.
TS16: 2nd branchial arch	Strong	2A -/-	Expression in this mutant is the same as in wild-type embryos.
TS16: 3rd branchial arch	Strong	2A -/-	Expression in this mutant is the same as in wild-type embryos.
TS16: 4th branchial arch	Strong	2A -/-	Expression in this mutant is the same as in wild-type embryos.

2B -/- ©

Specimen 2B -/-: embryonic day 9.5; Ednra^tm1Ywa/Ednra^tm1Ywa (more

)
Note: Theiler stage 16 was chosen for this 9.5dpc embryo because of the existence of the 4th branchial arch.

Structure	Level	Image	Note
TS16: 1st branchial arch ectoderm	Strong	2B -/-	Expression in this mutant is the same as in wild-type embryos.
TS16: 1st branchial arch mesenchyme	Strong	2B -/-	Expression in this mutant is the same as in wild-type embryos.
TS16: 2nd branchial arch	Strong	2B -/-	Expression in this mutant is the same as in wild-type embryos.
TS16: 3rd branchial arch	Strong	2B -/-	Expression in this mutant is the same as in wild-type embryos.
TS16: 4th branchial arch	Strong	2B -/-	Expression in this mutant is the same as in wild-type embryos.

2E +/+ ©

Specimen 2E +/+: embryonic day 10.5 (more

)

Structure	Level	Image	Note
TS16: 1st branchial arch	Present	2E +/+	The pattern is similar to that seen in 9.5dpc embryos.
TS16: 2nd branchial arch	Present	2E +/+	The pattern is similar to that seen in 9.5dpc embryos.
TS16: 3rd branchial arch	Present	2E +/+	The pattern is similar to that seen in 9.5dpc embryos.

2F +/+ ©

Specimen 2F +/+: embryonic day 10.5 (more

)

Structure	Level	Image	Note
TS17: 1st branchial arch	Present	2F +/+	The pattern is similar to that seen in 9.5dpc embryos.
TS17: 2nd branchial arch	Present	2F +/+	The pattern is similar to that seen in 9.5dpc embryos.
TS17: 3rd branchial arch	Present	2F +/+	The pattern is similar to that seen in 9.5dpc embryos.

2E -/- ©

Specimen 2E -/-: embryonic day 10.5; Ednra^tm1Ywa/Ednra^tm1Ywa (more

)

Structure	Level	Image
TS17: 1st branchial arch	Present	2E -/-
TS17: 3rd branchial arch	Present	2E -/-
TS17: 2nd branchial arch	Absent	2E -/-

2F -/- ©

Specimen 2F -/-: embryonic day 10.5; Ednra^tm1Ywa/Ednra^tm1Ywa (more

)

Structure	Level	Image
TS17: 1st branchial arch	Present	2F -/-
TS17: 3rd branchial arch	Present	2F -/-
TS17: 2nd branchial arch	Absent	2F -/-

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