Gene Expression Data
Assay Details
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Assay
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Reference:
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J:68216
Akasaka T, et al., Mice doubly deficient for the Polycomb Group genes Mel18 and Bmi1 reveal synergy and requirement for maintenance but not initiation of Hox gene expression. Development. 2001 May;128(9):1587-97
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1933569
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Gene symbol:
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Hoxb6
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Gene name:
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homeobox B6
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Probe:
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Hoxb6 probe5
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
3 C:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Pcgf2tm1Hko/Pcgf2tm1Hko
Bmi1tm1Brn/Bmi1tm1Brn
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future forebrain |
Present |
Regionally restricted |
3C
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The anterior boundary of expression was shifted up to the forebrain.
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| TS15: embryo |
Present |
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3C
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An overall reduction of expression in the presumptive trunk region.
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Specimen
3 D:
embryonic day 9.5
(more  )
Specimen
3 D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: neural tube |
Present |
Regionally restricted |
3D
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Expression was restricted to the presumptive cervical region of the neural tube.
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Specimen
3 L:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Pcgf2tm1Hko/Pcgf2tm1Hko
Bmi1tm1Brn/Bmi1tm1Brn
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: embryo |
Present |
Regionally restricted |
3L
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Expression in the caudal region of the embryo.
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Specimen
3 M:
embryonic day 8.5
(more )
Specimen
3 M:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: embryo |
Present |
Regionally restricted |
3M
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Pcgf2tm1Hko/Pcgf2tm1Hko
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: neural tube |
Present |
Regionally restricted |
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Expression in single mutants was restricted to the presumptive cervical region of the neural tube.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Bmi1tm1Brn/Bmi1tm1Brn
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: neural tube |
Present |
Regionally restricted |
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Expression in single mutants was restricted to the presumptive cervical region of the neural tube.
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Analysis Tools