Gene Expression Data
Assay Details
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Assay
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Reference:
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J:68215
Xue Y, et al., Mesodermal patterning defect in mice lacking the Ste20 NCK interacting kinase (NIK). Development. 2001 May;128(9):1559-72
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1933089
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Gene symbol:
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T
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Gene name:
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brachyury, T-box transcription factor T
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Probe:
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T cDNA9
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
3 B:
embryonic day 8.5
(more  )
Specimen
3 B:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3 F:
(close )
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Genetic Background:
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either: (involves: 129S/SvEv * Swiss Webster) or (involves: CD-1 * Swiss Webster)
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Mutant Allele(s):
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Map4k4tm1Eys/Map4k4tm1Eys
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: primitive streak |
Present |
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3F
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No expression in the large mass of cells adjacent to the primitive streak.
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Specimen
5 A) e:
embryonic day 7.5
(more )
Specimen
5 A) e:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5 A) g:
embryonic day 7.5
(more )
Note: Section from embryo in e.
Specimen
5 A) g:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Section from embryo in e.
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Specimen
5 A) f:
(close )
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Genetic Background:
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either: (involves: 129S/SvEv * Swiss Webster) or (involves: CD-1 * Swiss Webster)
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Mutant Allele(s):
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Map4k4tm1Eys/Map4k4tm1Eys
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Age:
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embryonic day 7.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: primitive streak |
Present |
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5A f
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Expression in the bulge of cells adjacent to the amniotic cavity along the proximodistal axis as well as in cells anterior to the primitive streak. The extra cells that accumulate in the primitive streak do not express T.
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Specimen
5 A) h:
(close )
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Genetic Background:
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either: (involves: 129S/SvEv * Swiss Webster) or (involves: CD-1 * Swiss Webster)
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Mutant Allele(s):
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Map4k4tm1Eys/Map4k4tm1Eys
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Age:
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embryonic day 7.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Section of embryo in f.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: primitive streak |
Present |
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5A h
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Expression in the bulge of cells adjacent to the amniotic cavity along the proximodistal axis as well as in cells anterior to the primitive streak. The extra cells that accumulate in the primitive streak do not express T.
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Specimen
5 A) i:
(close )
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Genetic Background:
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either: (involves: 129S/SvEv * Swiss Webster) or (involves: CD-1 * Swiss Webster)
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Mutant Allele(s):
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Map4k4tm1Eys/Map4k4tm1Eys
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Age:
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embryonic day 7.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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transverse section.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: amniotic cavity |
Present |
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5A i
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Expression in the bulge of cells adjacent to the amniotic cavity along the proximodistal axis as well as in cells anterior to the primitive streak. The extra cells that accumulate in the primitive streak do not express T.
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| TS11: primitive streak |
Present |
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5A i
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Expression in the bulge of cells adjacent to the amniotic cavity along the proximodistal axis as well as in cells anterior to the primitive streak. The extra cells that accumulate in the primitive streak do not express T.
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Analysis Tools