Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:25027
Hebert JM, et al., mRNA localization studies suggest that murine FGF-5 plays a role in gastrulation. Development. 1991 Jun;112(2):407-15
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:1930524
|
Gene symbol:
|
Fgf5
|
Gene name:
|
fibroblast growth factor 5
|
Probe:
|
Fgf-5 cDNA1
|
Probe preparation:
|
Antisense labelled with S35 RNA
|
Visualized with:
|
Autoradiography
|
|
Results
|
Specimen
1B,1C:
embryonic day 5.5
(more )
Note: Panes 1B and 1C are bright field and dark field views of the same specimen.
Specimen
1B,1C:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 5.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Panes 1B and 1C are bright field and dark field views of the same specimen.
|
Structure |
Level |
Pattern |
Image |
Note |
TS7: embryo |
Absent |
|
1B/C
|
Authors state that "in one of the embryos removed from the uterus during this period" (i.e. one less advanced in development), Fgf5 mRNA was not detectable.
|
Specimen
1E,1F:
embryonic day 6.5
(more )
Note: Panes 1E and 1F are bright field and dark field views of the same specimen.
Specimen
1E,1F:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 6.5
|
Age Note:
|
Theiler stage of this specimen assigned by curators from EMAGE.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Panes 1E and 1F are bright field and dark field views of the same specimen.
|
Structure |
Level |
Pattern |
Image |
Note |
TS8: epiblast |
Strong |
Uniform |
1E/F
|
Fgf5 mRNA was readily detectable at uniformly high levels throughout the embryonic ectoderm at this stage.
|
TS8: extraembryonic component |
Present |
Regionally restricted |
1E/F
|
Fgf5 mRNA was undetectable in the proximal extraembryonic tissue.
|
TS8: extraembryonic visceral endoderm |
Ambiguous |
|
1E/F
|
Authors state that it was difficult to determine whether Fgf5 mRNA was expressed in visceral endoderm cells.
|
Specimen
2C,2D:
embryonic day 7.0
(more )
Note: Panes 2C and 2D are bright field and dark field views of the same specimen.
Specimen
2C,2D:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 7.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Panes 2C and 2D are bright field and dark field views of the same specimen.
|
Structure |
Level |
Pattern |
Image |
Note |
TS10: embryo |
Present |
Regionally restricted |
2C/D
|
Expression of Fgf5 remained restricted to the embryonic portion of the conceptus.
|
TS10: embryo ectoderm |
Strong |
Graded |
2C/D
|
Levels of Fgf5 mRNA appeared to be relatively high throughout the embryonic ectoderm, with evidence of differential expression along the proximodistal axis; the signal intensity in the most proximal region was lower than in the most distal region.
|
TS10: embryo endoderm |
Strong |
Regionally restricted |
2C/D
|
Observed both Fgf5-expressing (likely definitive endoderm cells) and non-expressing cells in the endodermal cell layer. Distally, Fgf5 mRNA was detected a levels similar to those in adjacent embryonic ectoderm; proximally, no Fgf5 mRNA was detectable
|
TS10: embryo mesoderm |
Strong |
Regionally restricted |
2C/D
|
Fgf5 was not detected in cells that had migrated either proximally into the extraembryonic region or proximolaterally between embryonic ectoderm and visceral endoderm. Cells exiting the streak more distally expressed Fgf5 similarly to distal ectoderm.
|
TS10: primitive streak |
Strong |
Regionally restricted |
2C/D
|
Fgf5 mRNA levels were relatively high throughout the streak, with differential expression along the proximodistal axis; signal intensity in the most proximal region was 2- to 3-fold lower than in the most distal region.
|
TS10: extraembryonic component |
Absent |
|
2C/D
|
Fgf5 expression remained restricted to the embryonic portion of the conceptus.
|
Specimen
3B,3C:
embryonic day 7.5
(more )
Note: Panes 3B and 3C are bright field and dark field views of the same specimen.
Specimen
3B,3C:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 7.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Panes 3B and 3C are bright field and dark field views of the same specimen.
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: embryo ectoderm |
Strong |
Graded |
3B/C
|
Embryonic ectoderm expressed Fgf5 mRNA, with increasingly higher levels detectable towards the distal end.
|
TS11: embryo endoderm |
Strong |
|
3B/C
|
The endoderm surrounding the embryo, a mixed population of epiblast-derived and primitive endoderm-derived cells, contains levels of Fgf5 mRNA similar to those in adjacent mesoderm.
|
TS11: embryo mesoderm |
Strong |
Regionally restricted |
3B/C
|
No expression was detected in proximolateral mesoderm, whereas distal mesoderm was positive (roughly the same levels as in adjacent ectoderm). Head process cells 9cells that have migrated anterior to the distal end of the streak) were also positive.
|
TS11: allantois mesoderm |
Absent |
|
3B/C
|
No Fgf5 expression was detected in extraembryonic mesoderm.
|
TS11: amnion mesoderm |
Absent |
|
3B/C
|
No Fgf5 expression was detected in extraembryonic mesoderm.
|
TS11: chorion mesoderm |
Absent |
|
3B/C
|
No Fgf5 expression was detected in extraembryonic mesoderm.
|
TS11: yolk sac mesoderm |
Absent |
|
3B/C
|
No Fgf5 expression was detected in extraembryonic mesoderm.
|
Specimen
3D,3E:
embryonic day 7.75
(more )
Note: Panes 3D and 3E are bright field and dark field views of the same specimen.
Specimen
3D,3E:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 7.75
|
Age Note:
|
Theiler stage of this specimen assigned by curators from EMAGE.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Panes 3D and 3E are bright field and dark field views of the same specimen.
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: embryo |
Absent |
|
3D/E
|
Fgf5 mRNA was not detected in embryos at this stage (E7.75), as determined by serial section analysis through the embryo, although it was abundant in embryos from the same litter that were not as advanced in development
|
Specimen
not shown:
embryonic day 6.0
(more )
Specimen
not shown:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 6.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS8: epiblast |
Strong |
Uniform |
|
Expression was readily detectable at uniformly high levels throughout the embryonic ectoderm at this stage.
|
Specimen
not shown:
embryonic day 8.0
(more )
Specimen
not shown:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 8.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
|