Gene Expression Data
Assay Details
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Assay
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Reference:
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J:62028
Charite J, et al., The bHLH transcription factor dHAND controls Sonic hedgehog expression and establishment of the zone of polarizing activity during limb development. Development. 2000 Jun;127(11):2461-70
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1858450
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Gene symbol:
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Hand2
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Gene name:
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heart and neural crest derivatives expressed 2
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Probe:
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dhand probe16
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1. A.:
embryonic day 8.5
(more  )
Specimen
1. A.:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1. B.:
embryonic day 9.0
(more )
Specimen
1. B.:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1. C.:
embryonic day 9.5
(more )
Specimen
1. C.:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: forelimb bud |
Present |
Regionally restricted |
1C
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Expression detected in the posterior and flank of the right forelimb bud.
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Specimen
1. F.:
embryonic day 9.75
(more )
Specimen
1. F.:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.75
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: forelimb bud |
Strong |
Regionally restricted |
1F
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Expression detected in the posterior and of the right forelimb bud.
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Specimen
1. G.:
embryonic day 9.75
(more )
Specimen
1. G.:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.75
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: hindlimb bud |
Weak |
Regionally restricted |
1G
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Expression detected in center of right hindlimb bud.
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Specimen
1. H.:
embryonic day 10.25
(more )
Specimen
1. H.:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.25
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
1H
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Expression detected in the posterior of the right forelimb bud.
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Specimen
1. I.:
embryonic day 10.25
(more )
Specimen
1. I.:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.25
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud |
Present |
Regionally restricted |
1I
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Expression detected in the posterior of the right hindlimb bud.
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Specimen
1. K.:
embryonic day 10.75
(more )
Specimen
1. K.:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.75
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: forelimb bud |
Present |
Regionally restricted |
1K
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Strong expression detected in the posterior and weak in the anterior and proximal right forelimb bud.
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Specimen
1. M.:
embryonic day 10.75
(more )
Specimen
1. M.:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.75
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1. O:
embryonic day 11.5
(more )
Specimen
1. O:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
1O
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Strong expression detected in the posterior of the right forelimb. expression also detected in the proximal and anterior apical right forelimb bud.
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Specimen
1. P.:
embryonic day 11.5
(more )
Specimen
1. P.:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindlimb bud |
Present |
Regionally restricted |
1P
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Strong expression detected in the posterior of the right hindlimb. expression also detected in the proximal and anterior apical right hindlimb bud.
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Specimen
1. Q.:
embryonic day 12.5
(more )
Specimen
1. Q.:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forelimb |
Present |
Regionally restricted |
1Q
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Strong expression detected in the posterior of the right forelimb and weak expression also detected in the anterior.
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Specimen
E 7.5:
embryonic day 7.5
(more )
Specimen
E 7.5:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo |
Present |
Regionally restricted |
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expression detected in the proximal posterior of the embryo.
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Specimen
6. B.:
embryonic day 10.5
(more )
Specimen
6. B.:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
6B
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Expression detected in the posterior and flank of the right forelimb.
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Specimen
6. C.:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Weak |
Regionally restricted |
6C
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Expression detected in the posterior margin of the right forelimb.
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Specimen
6. D.:
embryonic day 11.5
(more )
Specimen
6. D.:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forelimb |
Present |
Regionally restricted |
6D
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Expression detected in the posterior of the right forelimb.
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Specimen
6. F.:
embryonic day 11.5; Dbf/Dbf +
(more )
Specimen
6. F.:
(close )
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Genetic Background:
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involves: 101/H * C3H/HeH
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Mutant Allele(s):
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Dbf/Dbf+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forelimb |
Present |
Regionally restricted |
6F
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Expression detected in the distal margin of the right forelimb.
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Analysis Tools