Bmp2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:60091 Moon AM, et al., Normal limb development in conditional mutants of Fgf4. Development. 2000 Mar;127(5):989-96
Assay type:	RNA in situ
MGI Accession ID:	MGI:1353487
Gene symbol:	Bmp2
Gene name:	bone morphogenetic protein 2

Probe:	Bmp2 probe6
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

5C F4^+/+ top ©

Specimen 5C F4+/+ top: embryonic day 10.5 (more

)

Structure	Level	Image
TS17: forelimb bud apical ectodermal ridge	Present	5C F4^+/+ top
TS17: hindlimb bud apical ectodermal ridge	Present	5C F4^+/+ top
TS17: limb mesenchyme	Present	5C F4^+/+ top

Specimen 5C F4+/+ bottom: embryonic day 11.5 (more

)

Structure	Level	Image
TS19: footplate apical ectodermal ridge	Present	5C F4^+/+ bottom
TS19: handplate apical ectodermal ridge	Present	5C F4^+/+ bottom
TS19: limb mesenchyme	Present	5C F4^+/+ bottom

5C F4^AP/N top ©

Specimen 5C F4^AP/N top: embryonic day 10.5; Fgf4^tm1Mrc/Fgf4^tm2Mrc (more

)

Structure	Level	Image	Note
TS17: forelimb bud apical ectodermal ridge	Present	5C F4^AP/N top	Expression is as in wild type.
TS17: hindlimb bud apical ectodermal ridge	Present	5C F4^AP/N top	Expression is as in wild type.
TS17: limb mesenchyme	Present	5C F4^AP/N top	Expression is as in wild type.

Specimen 5C F4^AP/N bottom: embryonic day 11.5; Fgf4^tm1Mrc/Fgf4^tm2Mrc (more

)

Structure	Level	Image	Note
TS19: footplate apical ectodermal ridge	Present	5C F4^AP/N bottom	Expression is as in wild type.
TS19: handplate apical ectodermal ridge	Present	5C F4^AP/N bottom	Expression is as in wild type.
TS19: limb mesenchyme	Present	5C F4^AP/N bottom	Expression is as in wild type.

Specimen 5C F4^AP/N;T top: embryonic day 10.5; Fgf4^tm1Mrc/Fgf4^tm2Mrc, Tg(Rarb-cre)1Mrc/0 (more

)
Note: Conditional mutant.

Structure	Level	Image	Note
TS17: forelimb bud apical ectodermal ridge	Present	5C F4^AP/N;T top	Expression is as in wild type.
TS17: hindlimb bud apical ectodermal ridge	Present	5C F4^AP/N;T top	Expression is as in wild type.
TS17: limb mesenchyme	Present	5C F4^AP/N;T top	Expression is as in wild type.

Specimen 5C F4^AP/N;T bottom: embryonic day 11.5; Fgf4^tm1Mrc/Fgf4^tm2Mrc, Tg(Rarb-cre)1Mrc/0 (more

)
Note: Conditional mutant.

Structure	Level	Image	Note
TS19: footplate apical ectodermal ridge	Present	5C F4^AP/N;T bottom	Expression is as in wild type.
TS19: handplate apical ectodermal ridge	Present	5C F4^AP/N;T bottom	Expression is as in wild type.
TS19: limb mesenchyme	Present	5C F4^AP/N;T bottom	Expression is as in wild type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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