Gene Expression Data
Assay Details
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Assay
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Reference:
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J:1975
Herrmann BG, Expression pattern of the Brachyury gene in whole-mount TWis/TWis mutant embryos. Development. 1991 Nov;113(3):913-7
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1345707
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Gene symbol:
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T
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Gene name:
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brachyury, T-box transcription factor T
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Probe:
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T cDNA9
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Probe preparation:
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Antisense labelled with biotin RNA
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Visualized with:
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Beta-galactosidase
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Results
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Specimen
not shown:
embryonic day 6.5
(more  )
Specimen
not shown:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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Not Specified
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Specimen
1A:
embryonic day 7.0
(more )
Note: No visible differences between normal and mutant embryos were revealed.
Specimen
1A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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No visible differences between normal and mutant embryos were revealed.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS10: embryo |
Present |
Regionally restricted |
1A
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Transcripts are detected in cells along the midline of the posterior half to the distal tip of the egg cylinder.
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| TS10: primitive streak |
Present |
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1A
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| TS10: embryo mesoderm |
Absent |
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1A
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Expression is down-regulated to undetectable levels in mesoderm cells migrating away from the streak.
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Specimen
not shown:
embryonic day 7.0; TWis/ TWis
(more )
Note: No visible differences between normal and mutant embryos were revealed.
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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TWis/TWis
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Age:
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embryonic day 7.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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No visible differences between normal and mutant embryos were revealed.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS10: embryo |
Present |
Regionally restricted |
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Expression in this mutant is the same as in wild-type embryos.
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| TS10: primitive streak |
Present |
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Expression in this mutant is the same as in wild-type embryos.
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| TS10: embryo mesoderm |
Absent |
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Expression in this mutant is the same as in wild-type embryos.
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Specimen
1B:
embryonic day 7.3
(more )
Note: Head process is present. No visible differences between normal and mutant embryos were revealed.
Specimen
1B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.3
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Head process is present. No visible differences between normal and mutant embryos were revealed.
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Specimen
not shown:
embryonic day 7.3; TWis/ TWis
(more )
Note: Head process is present. No visible differences between normal and mutant embryos were revealed.
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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TWis/TWis
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Age:
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embryonic day 7.3
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Head process is present. No visible differences between normal and mutant embryos were revealed.
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Specimen
1C:
embryonic day 7.5
(more )
Note: Amniotic cavity is present. No visible differences between normal and mutant embryos were revealed.
Specimen
1C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Amniotic cavity is present. No visible differences between normal and mutant embryos were revealed.
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Specimen
not shown:
embryonic day 7.5; TWis/ TWis
(more )
Note: Amniotic cavity is present. No visible differences between normal and mutant embryos were revealed.
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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TWis/TWis
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Amniotic cavity is present. No visible differences between normal and mutant embryos were revealed.
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Specimen
1D:
embryonic day 7.75
(more )
Note: Neural plate is present. No visible differences between normal and mutant embryos were revealed.
Specimen
1D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.75
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Neural plate is present. No visible differences between normal and mutant embryos were revealed.
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Specimen
not shown:
embryonic day 7.75; TWis/ TWis
(more )
Note: Neural plate is present. No visible differences between normal and mutant embryos were revealed.
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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TWis/TWis
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Age:
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embryonic day 7.75
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Neural plate is present. No visible differences between normal and mutant embryos were revealed.
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Specimen
1E:
embryonic day 8.0
(more )
Note: Neural folds are present.
Specimen
1E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Neural folds are present.
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Specimen
1F:
embryonic day 8.0; TWis/ TWis
(more )
Note: Neural folds are present. The archenteron/node is disrupted in the mutant.
Specimen
1F:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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TWis/TWis
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Neural folds are present. The archenteron/node is disrupted in the mutant.
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Specimen
1G:
embryonic day 8.0; TWis/ TWis
(more )
Note: Neural folds are present.
Specimen
1G:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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TWis/TWis
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Neural folds are present.
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Specimen
1H:
embryonic day 8.25
(more )
Specimen
1H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.25
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1I:
embryonic day 8.25; TWis/ TWis
(more )
Note: Mutant embryos display a kink in the streak posterior to the end of the notochord precursor.
Specimen
1I:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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TWis/TWis
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Age:
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embryonic day 8.25
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Mutant embryos display a kink in the streak posterior to the end of the notochord precursor.
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Specimen
1K right:
embryonic day 8.5
(more )
Note: 8 somite stage.
Specimen
1K right:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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8 somite stage.
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Specimen
1K left:
embryonic day 8.5; TWis/ TWis
(more )
Note: 8 somite stage. Mutant embryos display a kink in the streak posterior to the end of the notochord precursor.
Specimen
1K left:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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TWis/TWis
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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8 somite stage. Mutant embryos display a kink in the streak posterior to the end of the notochord precursor.
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Specimen
2A:
embryonic day 8.25
(more )
Specimen
2A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.25
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2B:
embryonic day 8.25
(more )
Specimen
2B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.25
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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