Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:39344
Yoshikawa Y, et al., Evidence that absence of Wnt-3a signaling promotes neuralization instead of paraxial mesoderm development in the mouse. Dev Biol. 1997 Mar 15;183(2):234-42
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:1338370
|
Gene symbol:
|
Ascl1
|
Gene name:
|
achaete-scute family bHLH transcription factor 1
|
Probe:
|
Mash1 cDNA3
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
Visualized with:
|
Alkaline phosphatase
|
|
Results
|
Specimen
5A:
embryonic day 9.5
(more )
Specimen
5A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
5B:
(close )
Genetic Background:
|
involves: 129S1/Sv
|
Mutant Allele(s):
|
Wnt3atm1Amc/Wnt3atm1Amc
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
5C:
embryonic day 9.5; Wnt3atm1Amc/ Wnt3atm1Amc
(more )
Note: Fig. 5C is anterior to the forelimb bud.
Specimen
5C:
(close )
Genetic Background:
|
involves: 129S1/Sv
|
Mutant Allele(s):
|
Wnt3atm1Amc/Wnt3atm1Amc
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Fig. 5C is anterior to the forelimb bud.
|
Specimen
5D:
embryonic day 9.5; Wnt3atm1Amc/ Wnt3atm1Amc
(more )
Note: Fig. 5D is posterior to the forelimb bud.
Specimen
5D:
(close )
Genetic Background:
|
involves: 129S1/Sv
|
Mutant Allele(s):
|
Wnt3atm1Amc/Wnt3atm1Amc
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Fig. 5D is posterior to the forelimb bud.
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: embryo |
Present |
Regionally restricted |
5D
|
Expression in the mutant is ectopically localized ventral to the original neural tube in the region posterior to the forelimbs.
|
|