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Gene Expression Data
Assay Details
Assay
Reference: J:30825 Sporle R, et al., Severe defects in the formation of epaxial musculature in open brain (opb) mutant mouse embryos. Development. 1996 Jan;122(1):79-86
Assay type: RNA in situ
MGI Accession ID: MGI:1336670
Gene symbol: Wnt3a
Gene name: wingless-type MMTV integration site family, member 3A
Probe: Wnt3a probe2
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 1G: embryonic day 10.5 (more )
Note: Double labeled: purple - Wnt3a; purple - Myf5 (assay MGI:1336630).
Structure Level Pattern Image Note
TS17: neural tube roof plate Present 1G
TS17: future spinal cord Present Regionally restricted 1G Expression is in the dorsal-most area of the future spinal chord.
Specimen 1H: embryonic day 10.5; Rab23opb/Rab23opb (more )
Note: Double labeled: purple - Wnt3a; purple - Myf5 (assay MGI:1336630).
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 1H Expression in the mutant is narrow, patchy and irregular.
Specimen 1I: embryonic day 10.5 (more )
Note: Double labeled: purple - Wnt3a; purple - Myf5 (assay MGI:1336630).
Structure Level Pattern Image Note
TS17: neural tube roof plate Present 1I
TS17: future spinal cord Present Regionally restricted 1I Expression is in the dorsal-most area of the future spinal chord.
Specimen 1K: embryonic day 10.5; Rab23opb/Rab23opb (more )
Note: Double labeled: purple - Wnt3a; purple - Myf5 (assay MGI:1336630).
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 1K Expression in the mutant is narrow, patchy and irregular.
Specimen 1L: embryonic day 13.0 (more )
Structure Level Pattern Image Note
TS21: spinal cord roof plate Present 1L
TS21: spinal cord Present Regionally restricted 1L Expression is in the dorsal spinal cord.
Specimen 1M: embryonic day 13.0; Rab23opb/Rab23opb (more )
Structure Level Pattern Image Note
TS21: spinal cord Present Regionally restricted 1M There are several gaps in expression in mutant embryos.
TS21: spinal cord roof plate Present Regionally restricted 1M There are several gaps in expression in mutant embryos.
Specimen 5E: embryonic day 9.5 (more )
Note: Double labeled: purple - Wnt3a; purple - Myf5 (assay MGI:1336630).
Structure Level Pattern Image Note
TS15: neural tube Present Regionally restricted 5E Expression is in the dorsal spinal cord.
Specimen 5F: embryonic day 9.5; Rab23opb/Rab23opb (more )
Note: Double labeled: purple - Wnt3a; purple - Myf5 (assay MGI:1336630).
Structure Level Pattern Image Note
TS15: neural tube Present Regionally restricted 5F Expression in the mutant is much narrower and patchy compared to wild type embryos and a few gaps were apparent.
Specimen 5G: embryonic day 9.5 (more )
Note: Double labeled: purple - Wnt3a; purple - Myf5 (assay MGI:1336630).
Structure Level Pattern Image Note
TS15: neural tube Present Regionally restricted 5G Expression is in the dorsal spinal cord.
Specimen 5H: embryonic day 9.5; Rab23opb/Rab23opb (more )
Note: Double labeled: purple - Wnt3a; purple - Myf5 (assay MGI:1336630).
Structure Level Pattern Image Note
TS15: neural tube Present Regionally restricted 5H Expression in the mutant is much narrower and patchy compared to wild type embryos and a few gaps were apparent.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory