Results
|
Specimen
1A,C:
embryonic day 10.5
(more )
Specimen
1A,C:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: myotome |
Present |
Regionally restricted |
1A
,
1C
|
Expression is in both dorsal and ventral myotome regions including the somitic bud.
|
TS17: somite |
Present |
Regionally restricted |
1A
,
1C
|
Expression is in both dorsal and ventral myotome regions including the somitic bud.
|
Specimen
1G:
embryonic day 10.5
(more )
Note: Double labeled: purple - Myf5; purple - Wnt3a (assay MGI:1336670).
Specimen
1G:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: purple - Myf5; purple - Wnt3a (assay MGI:1336670).
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: myotome |
Present |
Regionally restricted |
1G
|
Expression is in both dorsal and ventral myotome regions including the somitic bud.
|
TS17: somite |
Present |
Regionally restricted |
1G
|
Expression is in both dorsal and ventral myotome regions including the somitic bud.
|
Specimen
1I:
embryonic day 10.5
(more )
Note: Double labeled: purple - Myf5; purple - Wnt3a (assay MGI:1336670).
Specimen
1I:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: purple - Myf5; purple - Wnt3a (assay MGI:1336670).
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: myotome |
Present |
Regionally restricted |
1I
|
Expression is in both dorsal and ventral myotome regions including the somitic bud.
|
TS17: somite |
Present |
Regionally restricted |
1I
|
Expression is in both dorsal and ventral myotome regions including the somitic bud.
|
Specimen
1B,D:
(close )
Genetic Background:
|
involves: C57BL/6 * NMRI
|
Mutant Allele(s):
|
Rab23opb/Rab23opb
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: somite 12 |
Present |
Regionally restricted |
1D
,
1B
|
Expression is strongly reduced in the anterodorsal and posterodorsal regions of each somite, resulting in a flame-like appearance of the remaining dorsal myotome.
|
TS17: somite 13 |
Present |
Regionally restricted |
1D
,
1B
|
Expression is strongly reduced in the anterodorsal and posterodorsal regions of each somite, resulting in a flame-like appearance of the remaining dorsal myotome.
|
TS17: somite 14 |
Present |
Regionally restricted |
1D
,
1B
|
Expression is strongly reduced in the anterodorsal and posterodorsal regions of each somite, resulting in a flame-like appearance of the remaining dorsal myotome.
|
TS17: somite 15 |
Present |
Regionally restricted |
1D
,
1B
|
Expression is strongly reduced in the anterodorsal and posterodorsal regions of each somite, resulting in a flame-like appearance of the remaining dorsal myotome.
|
TS17: somite 16 |
Present |
Regionally restricted |
1D
,
1B
|
Expression is strongly reduced in the anterodorsal and posterodorsal regions of each somite, resulting in a flame-like appearance of the remaining dorsal myotome.
|
TS17: somite 17 |
Present |
Regionally restricted |
1D
,
1B
|
Expression is strongly reduced in the anterodorsal and posterodorsal regions of each somite, resulting in a flame-like appearance of the remaining dorsal myotome.
|
TS17: myotome |
Present |
Regionally restricted |
1B
,
1D
|
In lower thoracic and lumbar axial regions almost no expression could be detected in dorsal myotome regions. Expression in ventral myotome regions, appeared normal. In cervical regions, the cap-like dorsal-most expression observed is missing.
|
Specimen
1H:
(close )
Genetic Background:
|
involves: C57BL/6 * NMRI
|
Mutant Allele(s):
|
Rab23opb/Rab23opb
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: purple - Myf5; purple - Wnt3a (assay MGI:1336670).
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: myotome |
Present |
Regionally restricted |
1H
|
Defects noted in myotome formation were detectable within the same axial regions as the abnormal Wnt3a expression in the neural tube.
|
Specimen
1K:
(close )
Genetic Background:
|
involves: C57BL/6 * NMRI
|
Mutant Allele(s):
|
Rab23opb/Rab23opb
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: purple - Myf5; purple - Wnt3a (assay MGI:1336670).
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: myotome |
Present |
Regionally restricted |
1K
|
Defects noted in myotome formation were detectable within the same axial regions as the abnormal Wnt3a expression in the neural tube.
|
Specimen
not shown:
embryonic day 12.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
Not Specified
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3G:
embryonic day 11.5
(more )
Specimen
3G:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3H:
(close )
Genetic Background:
|
involves: C57BL/6 * NMRI
|
Mutant Allele(s):
|
Rab23opb/Rab23opb
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: body wall mesenchyme |
Weak |
Regionally restricted |
3H
|
In mutants, expression shows that ventral muscle anlagen is not impaired, but dorsal muscle anlagen is severely reduced.
|
Specimen
3I:
embryonic day 13.0
(more )
Note: 3I is from upper thoracic level.
Specimen
3I:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 13.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
3I is from upper thoracic level.
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: skeletal muscle |
Present |
Regionally restricted |
3I
|
In cross-sections of the upper thoracic regions, expression was detected in the developing musculature.
|
Specimen
3K:
embryonic day 13.0; Rab23opb/ Rab23opb
(more )
Note: 3K is from upper thoracic level.
Specimen
3K:
(close )
Genetic Background:
|
involves: C57BL/6 * NMRI
|
Mutant Allele(s):
|
Rab23opb/Rab23opb
|
Age:
|
embryonic day 13.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
3K is from upper thoracic level.
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: skeletal muscle |
Present |
Regionally restricted |
3K
|
In cross-sections of upper thoracic regions from mutant embryos, remnants of dorsal muscle could be observed.
|
TS21: trunk mesenchyme |
Present |
Regionally restricted |
3K
|
Patches of expression were detected in the loose dorsal mesenchyme.
|
Specimen
3L:
embryonic day 13.0
(more )
Note: 3L is from lower thoracic level.
Specimen
3L:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 13.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
3L is from lower thoracic level.
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: skeletal muscle |
Present |
Regionally restricted |
3L
|
In cross-sections of the lower thoracic regions, expression was detected in the developing musculature.
|
Specimen
3M:
embryonic day 13.0; Rab23opb/ Rab23opb
(more )
Note: 3M is from lower thoracic level.
Specimen
3M:
(close )
Genetic Background:
|
involves: C57BL/6 * NMRI
|
Mutant Allele(s):
|
Rab23opb/Rab23opb
|
Age:
|
embryonic day 13.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
3M is from lower thoracic level.
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: skeletal muscle |
Present |
Regionally restricted |
3M
|
In cross-sections of lower thoracic regions from mutant embryos, there was almost no dorsal muscle.
|
TS21: trunk mesenchyme |
Present |
Regionally restricted |
3M
|
Patches of expression were detected in the loose dorsal mesenchyme.
|
Specimen
5E:
embryonic day 9.5
(more )
Note: Double labeled: purple - Myf5; purple - Wnt3a (assay MGI:1336670).
Specimen
5E:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: purple - Myf5; purple - Wnt3a (assay MGI:1336670).
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: myotome |
Present |
Regionally restricted |
5E
|
In advanced somites, expression is in a triangular shaped pattern of positive cells in the dorsal myotome.
|
TS15: somite |
Present |
Regionally restricted |
5E
|
In newly generated somites, expression is in a band of cells in the dorsomedial lip.
|
Specimen
5F:
(close )
Genetic Background:
|
involves: C57BL/6 * NMRI
|
Mutant Allele(s):
|
Rab23opb/Rab23opb
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: purple - Myf5; purple - Wnt3a (assay MGI:1336670).
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: myotome |
Present |
Regionally restricted |
5F
|
In mutant embryos, only a central domain of expressing cells is seen in the dorsal myotome, such that large gaps in expression are apparent. The dorsomedial expression is missing.
|
TS15: somite |
Present |
Regionally restricted |
5F
|
In mutant embryos, only a central domain of expressing cells is seen in the dorsal myotome, such that large gaps in expression are apparent. The dorsomedial expression is missing.
|
Specimen
5G:
embryonic day 9.5
(more )
Note: Double labeled: purple - Myf5; purple - Wnt3a (assay MGI:1336670).
Specimen
5G:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: purple - Myf5; purple - Wnt3a (assay MGI:1336670).
|
Specimen
5H:
(close )
Genetic Background:
|
involves: C57BL/6 * NMRI
|
Mutant Allele(s):
|
Rab23opb/Rab23opb
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: purple - Myf5; purple - Wnt3a (assay MGI:1336670).
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: myotome |
Present |
Regionally restricted |
5H
|
Aberrant expression was visible in the developing dorsal myotomes along the same axial regions where the neural tube abnormalities occurred.
|
|