Gene Expression Data
Assay Details
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Assay
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Reference:
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J:42110
Wassarman KM, et al., Specification of the anterior hindbrain and establishment of a normal mid/hindbrain organizer is dependent on Gbx2 gene function. Development. 1997 Aug;124(15):2923-34
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1331435
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Gene symbol:
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Otx2
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Gene name:
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orthodenticle homeobox 2
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Probe:
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Otx2 cDNA1
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
4M:
embryonic day 12.5
(more  )
Specimen
4M:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: embryo |
Present |
Regionally restricted |
4M
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Specimen
4N:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2J) or (involves: 129S1/Sv * 129X1/SvJ * FVB/N)
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Mutant Allele(s):
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Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: cerebellum primordium |
Present |
Regionally restricted |
4N
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In mutants the expression domain extends caudally through the midbrain/hindbrain junction and into the region where the cerebellar anlage is found.
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| TS20: hindbrain |
Present |
Regionally restricted |
4N
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In mutants the expression domain extends caudally through the midbrain/hindbrain junction and into the region where the cerebellar anlage is found.
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| TS20: midbrain |
Present |
Regionally restricted |
4N
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In mutants the expression domain extends caudally through the midbrain/hindbrain junction and into the region where the cerebellar anlage is found.
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| TS20: embryo |
Present |
Regionally restricted |
4N
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In mutant embryos, caudal to the region in which expression occurs, ectopic expression is frequently detected laterally.
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Analysis Tools