Probe:	delta-EF1 probe 2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS12: head	Strong		2A	Strong expression was detected in the headfold.
TS12: lateral plate mesoderm	Moderate		2A
TS12: trunk unsegmented mesenchyme	Moderate		2A

Structure	Level	Pattern	Image	Note
TS15: branchial arch	Present		2B	Expression in derivatives of cranial neural crest, such as branchial arches and cranial ganglia.
TS15: cranial preganglion	Present		2B	Expression in derivatives of cranial neural crest, such as branchial arches and cranial ganglia.
TS15: somite	Moderate	Uniform	2B
TS15: hindlimb ridge	Strong		2B
TS15: forelimb bud	Strong		2B

Structure	Level	Pattern	Image	Note
TS15: hindlimb ridge	Strong	Uniform	2C	Expressed evenly in the entire limb.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Present	Regionally restricted	2D	Expression was confined to the posterior portion.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Regionally restricted	2E	Expression in the limb was restricted to the distal-posterior region.

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present		2G
TS19: trunk myotome	Present	Regionally restricted	2G	Expression was particularly high in the lateral myotome.
TS19: future arm mesenchyme	Present	Regionally restricted	2G	Myotomal expression was maintained in those cells that migrated in the limb.

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present		2H	Expression in the neural tube was clearly detected.
TS19: neural tube ventricular layer	Present	Regionally restricted	2H	Expression in the neural tube was particularly prominent in the ventricular zone.
TS19: dorsal root ganglion	Present		2H
TS19: trunk myotome	Present	Regionally restricted	2H	Expression was particularly high in the lateral myotome.

Structure	Level	Pattern	Image	Note
TS20: forelimb mesenchyme	Present	Regionally restricted	2I	Myotomal expression was maintained in those cells that migrated in the limb.
TS20: forelimb pre-cartilage condensation	Absent		2I	Expression was lost from the condensation of mesenchyme which gives rise to cartilaginous skeleton.

Structure	Level	Pattern	Image	Note
TS21: hindlimb interdigital region mesenchyme	Present		2J

Structure	Level	Pattern	Image	Note
TS21: hindlimb mesenchyme	Present	Regionally restricted	2K	Expression was detected in the perichondrium of the cartilage when the skeletal pattern was already formed.

Structure	Level	Pattern	Image	Note
TS22: forelimb	Present	Regionally restricted	2L	Expression was detected in the perichondrium of the cartilage when the skeletal pattern was already formed.