Probe:	Bmp4 cDNA5
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent		1B	Data is from an anterior section, a whole mount shows expression in more posterior sections.

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent		1G	Data is from an anterior section, a whole mount shows expression in more posterior sections.

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	1M	Expression is restricted to the anterior dorsal neurectoderm.
TS13: extraembryonic component	Present	Regionally restricted	1M	Expression is detected in the amnion.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present	Regionally restricted	2B	Expression is detected in the dorsal forebrain.

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Present	Regionally restricted	2H	Expression detected in the anteriormost dorsal roof of the telencephalon.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	3A	Expression is detected in the dorsomedial part of the telencephalon and the dorsalmidline of the anterior diencephalon. Transcripts are also detected in the dorsal hindbrain.
TS17: hindbrain	Present	Regionally restricted	3A	Expression is detected in the dorsomedial part of the telencephalon and the dorsalmidline of the anterior diencephalon. Transcripts are also detected in the dorsal hindbrain.
TS17: telencephalon	Present	Regionally restricted	3A	Expression is detected in the dorsomedial part of the telencephalon and the dorsalmidline of the anterior diencephalon. Transcripts are also detected in the dorsal hindbrain.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	4A	Expression is detected in the dorsomedial telencephalon, the dorsal diencephalon and the roofplate of the hindbrain.
TS19: metencephalon roof	Present	Regionally restricted	4A	Expression is detected in the dorsomedial telencephalon, the dorsal diencephalon and the roofplate of the hindbrain.
TS19: myelencephalon roof plate	Present	Regionally restricted	4A	Expression is detected in the dorsomedial telencephalon, the dorsal diencephalon and the roofplate of the hindbrain.
TS19: telencephalon	Present	Regionally restricted	4A	Expression is detected in the dorsomedial telencephalon, the dorsal diencephalon and the roofplate of the hindbrain.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	4F	Expression is detected in the roof between the cerebral hemispheres.

Structure	Level	Pattern	Image	Note
TS21: 3rd ventricle	Present	Regionally restricted	4K	Expression is detected in the medial walls of the lateral ventricle, the prospective hippocampus.
TS21: 4th ventricle	Present	Regionally restricted	4K	Expression is detected in the medial walls of the lateral ventricle, the prospective hippocampus.
TS21: lateral ventricle	Present	Regionally restricted	4K	Expression is detected in the medial walls of the lateral ventricle, the prospective hippocampus.
TS21: lateral ventricle choroid plexus	Strong	Uniform	4K
TS21: metencephalon part of 4th ventricle choroid plexus	Strong	Uniform	4K

Structure	Level	Pattern	Image	Note
TS16: future brain	Present		5J	Expression pattern is similar to Hfh4.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Regionally restricted	6C	Expression is detected in the dorsal midline and dorsomedial region and no expression is detected in the lateral telencephalon.