Gene Expression Data
Assay Details
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Assay
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Reference:
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J:25248
Conlon RA, et al., Notch1 is required for the coordinate segmentation of somites. Development. 1995 May;121(5):1533-45
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:1276151
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Gene symbol:
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Nefm
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Gene name:
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neurofilament, medium polypeptide
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Antibody:
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anti-neurofilament clone 2H3
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Assay notes:
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Goat anti-mouse IgG used as the secondary antibody at 1:500 dilution.
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Results
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Specimen
3A:
embryonic day 9.5
(more  )
Note: 20-somite embryo.
Specimen
3A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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20-somite embryo.
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Specimen
3B:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) or (involves: 129S1/Sv * 129X1/SvJ * CD-1)
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Mutant Allele(s):
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Notch1tm1Con/Notch1tm1Con
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: central nervous system |
Present |
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3B
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Positive cells were detected in the midbrain, and in condensations at the normal positions of the fifth and seventh cranial ganglia. The pattern of staining was similar to the 9.5-day wild-type embryo shown in Fig. 3A.
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| TS16: future midbrain |
Present |
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3B
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Positive cells were detected in the midbrain, and in condensations at the normal positions of the fifth and seventh cranial ganglia. The pattern of staining was similar to the 9.5-day wild-type embryo shown in Fig. 3A.
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Analysis Tools