Gene Expression Data
Assay Details
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Assay
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Reference:
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J:296111
Gao Z, et al., Zbed3 participates in the subcortical maternal complex and regulates the distribution of organelles. J Mol Cell Biol. 2018 Feb 1;10(1):74-88
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:8447801
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Gene symbol:
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Zbed3
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Gene name:
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zinc finger, BED type containing 3
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Results
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Specimen
1C GV:
postnatal adult
(more  )
Note: Double labeled: green - Zbed3; red - Nlrp5 (assay MGI:8447802).
Specimen
1C GV:
(close  )
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Genetic Background:
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CD-1
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Zbed3; red - Nlrp5 (assay MGI:8447802).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: primary oocyte |
Present |
Regionally restricted |
1C GV Merge
,
1C GV Zbed3
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Diffuse expression in cytoplasm, partially co-localized with Nlrp5 in cytoplasmic regions..
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Specimen
1C GV inset:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Zbed3tm1.1Lil/Zbed3tm1.1Lil
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Specimen
1C MII:
postnatal adult
(more )
Note: Double labeled: green - Zbed3; red - Nlrp5 (assay MGI:8447802).
Specimen
1C MII:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Zbed3; red - Nlrp5 (assay MGI:8447802).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: secondary oocyte |
Present |
Regionally restricted |
1C MII Zbed3
,
1C MII Merge
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Expression gradually concentrated in subcortex, partially co-localized with Nlrp5 in subcortical regions.
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Specimen
2C:
postnatal adult
(more )
Specimen
2C:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: oocyte |
Present |
Regionally restricted |
2C Zbed3/DNA
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2C Zbed3
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Expression restricted to cytoplasm of oocytes.
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| TS28: ovary |
Present |
Regionally restricted |
2C Zbed3/DNA
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2C Zbed3
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Expression restricted to cytoplasm of oocytes.
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Specimen
2F 1-Cell:
embryonic day 0.5
(more )
Note: Double labeled: green - Zbed3; red - F-actin.
Specimen
2F 1-Cell:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Zbed3; red - F-actin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS1: 1-cell stage embryo |
Present |
Regionally restricted |
2F 1-Cell Zbed3
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2F 1-Cell merged
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Specimen
2F 2-Cell:
embryonic day 1.0
(more )
Note: Double labeled: green - Zbed3; red - F-actin.
Specimen
2F 2-Cell:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Zbed3; red - F-actin.
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Specimen
2F Morula:
embryonic day 3.0
(more )
Note: Double labeled: green - Zbed3; red - F-actin.
Specimen
2F Morula:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 3.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Zbed3; red - F-actin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: compacted morula |
Present |
Regionally restricted |
2F Morula Zbed3
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2F Morula merged
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Expression localized in cytoplasm and concentrated in subcortex of morula
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Specimen
2F Blastocyst:
embryonic day 3.5
(more )
Note: Double labeled: green - Zbed3; red - F-actin.
Specimen
2F Blastocyst:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Zbed3; red - F-actin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: blastocyst |
Trace |
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2F Blastocyst merged
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2F Blastocyst Zbed3
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Specimen
6C WT:
postnatal adult
(more )
Specimen
6C WT:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Specimen
6C Mater tm/tm:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Nlrp5tm1Zbt/Nlrp5tm1Zbt
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: secondary oocyte |
Weak |
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6C Mater tm/tm
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Expression less abundant of Nlrp5-null MII eggs than in wild type and Khdc3-null.
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Specimen
6C Tle6 tm/tm:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Tle6tm1Lil/Tle6tm1Lil
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: secondary oocyte |
Trace |
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6C Tie6 tm/tm
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Expression less abundant of Tle6-null MII eggs than in wild type and Khdc3-null.
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Specimen
6C Filia tm/tm:
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Khdc3tm1Dean/Khdc3tm1Dean
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Specimen
6E Zbed3 +/delta (left):
postnatal adult; Zbed3tm1.1Lil/Zbed3 +
(more )
Specimen
6E Zbed3 +/delta (left):
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Zbed3tm1.1Lil/Zbed3+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: oocyte |
Present |
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6E Zbed3 Zbed3 +/delta (left)
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Specimen
6E Zbed3 delta/delta (left):
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Zbed3tm1.1Lil/Zbed3tm1.1Lil
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: oocyte |
Absent |
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6E Zbed3 Zbed3 delta/delta (left)
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Specimen
6E Zbed3 +/delta (right):
postnatal adult; Zbed3tm1.1Lil/Zbed3 +
(more )
Specimen
6E Zbed3 +/delta (right):
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Zbed3tm1.1Lil/Zbed3+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: oocyte |
Present |
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6E Zbed3 Zbed3 +/delta (right)
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Specimen
6E Zbed3 delta/delta (right):
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Zbed3tm1.1Lil/Zbed3tm1.1Lil
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: oocyte |
Absent |
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6E Zbed3 Zbed3 delta/delta (right)
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Analysis Tools