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Gene Expression Data
Assay Details
Assay
Reference: J:306379 Mohad V, et al., Inactivation of Lats1 and Lats2 highlights the role of hippo pathway effector YAP in larynx and vocal fold epithelium morphogenesis. Dev Biol. 2021 May;473:33-49
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7861495
Gene symbol: Krt8
Gene name: keratin 8
Antibody: anti-cytokeratin 8 [EP1628Y] (ab192467)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 2A: embryonic day 10.5 (more )
Note: Double labeled: green - Krt8; red - Krt5 (assay MGI:7861494).
Structure Level Pattern Image Note
TS17: respiratory tract epithelium Strong Regionally restricted 2A Strong expression in pseudostratified columnar epithelial progenitors in laryngopharyngeal region.

Specimen 2E: embryonic day 11.5 (more )
Note: Double labeled: green - Krt8; red - Krt5 (assay MGI:7861494).
Structure Level Pattern Image Note
TS19: respiratory tract epithelium Strong Regionally restricted 2E Strong expression in single layer of cuboidal epithelial progenitors in vocal fold lamina.

Specimen 2I: embryonic day 13.5 (more )
Note: Double labeled: green - Krt8; red - Krt5 (assay MGI:7861494).
Structure Level Pattern Image Note
TS21: larynx Present Regionally restricted 2I Expression in epithelial lamina of vocal fold.

Specimen 2M: embryonic day 15.5 (more )
Note: Double labeled: green - Krt8; red - Krt5 (assay MGI:7861494).
Structure Level Pattern Image Note
TS23: larynx Present Regionally restricted 2M Expression primarily in apical cell layer.

Specimen 2Q: embryonic day 16.5 (more )
Note: Double labeled: green - Krt8; red - Krt5 (assay MGI:7861494).
Structure Level Pattern Image Note
TS24: larynx Present Regionally restricted 2Q Expression primarily in apical cell layer.

Specimen 2U: embryonic day 18.5 (more )
Note: Double labeled: green - Krt8; red - Krt5 (assay MGI:7861494).
Structure Level Pattern Image Note
TS26: vocal folds Ambiguous 2U Expression almost disappears from apical layers in postnatal stages and adulthood.

Specimen adult not shown: postnatal adult (more )
Structure Level Pattern Image Note
TS28: vocal folds Ambiguous Expression almost disappears from apical layers in postnatal stages and adulthood.

Specimen 8A,B: postnatal day 0; Lats1tm1.1Jfm/Lats1+, Lats2tm1.1Jfm/Lats2+, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS27: vocal folds Strong Regionally restricted 8B , 8A Strong uniform expression expression in apical layer composed of suprabasal cells. No expression in basal layer. No expression in mesenchyme.

Specimen 8E,F: postnatal day 0; Lats1tm1.1Jfm/Lats1tm1.2Jfm, Lats2tm1.1Jfm/Lats2tm1.2Jfm, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS27: vocal folds epithelial cell Strong Regionally restricted 8F , 8E Strong expression in all epithelial cells. Exprssion in single layer of progenitors lining partially open vocal fold, and multilayered epitheliujm obstructing vocal fold lumen. Expression also present in mesenchyme.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory