Gene Expression Data
Assay Details
Assay
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Reference:
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J:306379
Mohad V, et al., Inactivation of Lats1 and Lats2 highlights the role of hippo pathway effector YAP in larynx and vocal fold epithelium morphogenesis. Dev Biol. 2021 May;473:33-49
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7861495
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Gene symbol:
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Krt8
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Gene name:
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keratin 8
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Results
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Specimen
2A:
embryonic day 10.5
(more )
Note: Double labeled: green - Krt8; red - Krt5 (assay MGI:7861494).
Specimen
2A:
(close )
Genetic Background:
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FVB/N
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Krt8; red - Krt5 (assay MGI:7861494).
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: respiratory tract epithelium |
Strong |
Regionally restricted |
2A
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Strong expression in pseudostratified columnar epithelial progenitors in laryngopharyngeal region.
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Specimen
2E:
embryonic day 11.5
(more )
Note: Double labeled: green - Krt8; red - Krt5 (assay MGI:7861494).
Specimen
2E:
(close )
Genetic Background:
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FVB/N
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Krt8; red - Krt5 (assay MGI:7861494).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: respiratory tract epithelium |
Strong |
Regionally restricted |
2E
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Strong expression in single layer of cuboidal epithelial progenitors in vocal fold lamina.
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Specimen
2I:
embryonic day 13.5
(more )
Note: Double labeled: green - Krt8; red - Krt5 (assay MGI:7861494).
Specimen
2I:
(close )
Genetic Background:
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FVB/N
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
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Note:
|
Double labeled: green - Krt8; red - Krt5 (assay MGI:7861494).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: larynx |
Present |
Regionally restricted |
2I
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Expression in epithelial lamina of vocal fold.
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Specimen
2M:
embryonic day 15.5
(more )
Note: Double labeled: green - Krt8; red - Krt5 (assay MGI:7861494).
Specimen
2M:
(close )
Genetic Background:
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FVB/N
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Krt8; red - Krt5 (assay MGI:7861494).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: larynx |
Present |
Regionally restricted |
2M
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Expression primarily in apical cell layer.
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Specimen
2Q:
embryonic day 16.5
(more )
Note: Double labeled: green - Krt8; red - Krt5 (assay MGI:7861494).
Specimen
2Q:
(close )
Genetic Background:
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FVB/N
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Krt8; red - Krt5 (assay MGI:7861494).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: larynx |
Present |
Regionally restricted |
2Q
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Expression primarily in apical cell layer.
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Specimen
2U:
embryonic day 18.5
(more )
Note: Double labeled: green - Krt8; red - Krt5 (assay MGI:7861494).
Specimen
2U:
(close )
Genetic Background:
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FVB/N
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Krt8; red - Krt5 (assay MGI:7861494).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: vocal folds |
Ambiguous |
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2U
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Expression almost disappears from apical layers in postnatal stages and adulthood.
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Specimen
adult not shown:
postnatal adult
(more )
Specimen
adult not shown:
(close )
Genetic Background:
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FVB/N
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS28: vocal folds |
Ambiguous |
|
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Expression almost disappears from apical layers in postnatal stages and adulthood.
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Specimen
8A,B:
(close )
Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6 * FVB/N
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Mutant Allele(s):
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Lats1tm1.1Jfm/Lats1+ Lats2tm1.1Jfm/Lats2+ Shhtm1(EGFP/cre)Cjt/Shh+
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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Structure |
Level |
Pattern |
Image |
Note |
TS27: vocal folds |
Strong |
Regionally restricted |
8B
,
8A
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Strong uniform expression expression in apical layer composed of suprabasal cells. No expression in basal layer. No expression in mesenchyme.
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Specimen
8E,F:
(close )
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: vocal folds |
epithelial cell |
Strong |
Regionally restricted |
8F
,
8E
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Strong expression in all epithelial cells. Exprssion in single layer of progenitors lining partially open vocal fold, and multilayered epitheliujm obstructing vocal fold lumen. Expression also present in mesenchyme.
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