Fgfr2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:90391 Yu K, et al., Conditional inactivation of FGF receptor 2 reveals an essential role for FGF signaling in the regulation of osteoblast function and bone growth. Development. 2003 Jul;130(13):3063-74
Assay type:	RNA in situ
MGI Accession ID:	MGI:7855546
Gene symbol:	Fgfr2
Gene name:	fibroblast growth factor receptor 2

Probe:	Fgfr2-TK probe
Probe preparation:	Antisense labelled with P33 RNA
Visualized with:	Autoradiography
Assay notes:	The probe detects the tyrosine kinase domains, outside of the deleted exons in the mutant.

Results

1H ©

Specimen 1H: embryonic day 16.5 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS24: perichondrium	preosteoblast	Present		1H
TS24: periosteum	preosteoblast	Present		1H
TS24: femur		Weak	Regionally restricted	1H	Weakly expressed in endosteal tissue and trabecular bone.

1K ©

Specimen 1K: postnatal day 7 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: perichondrium	preosteoblast	Present		1K
TS28: periosteum	preosteoblast	Present		1K
TS28: femur metaphysis	femoral osteoblast	Present		1K
TS28: femur diaphysis		Present	Regionally restricted	1K	Expressed in diaphyseal lacunae.
TS28: trabecular bone		Weak		1K
TS28: femur		Weak	Regionally restricted	1K	Weakly expressed in endosteal tissue.

3D ©

Specimen 3D: embryonic day 16.5 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS24: femur	periarticular chondrocyte	Present		3D
TS24: epiphyseal plate	growth plate cartilage chondrocyte	Absent		3D

3E ©

Specimen 3E: embryonic day 16.5; Fgfr2^tm1Dor/Fgfr2^tm1.1Dor, Twist2^tm1(cre)Dor/Twist2⁺ (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: femur	periarticular chondrocyte	Present		3E	Similar as in the control.

Specimen not shown: embryonic day 16.5 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS24: suture	osteoblast	Present	Regionally restricted		Expressed in osteoblasts in the osteogenic fronts.

Specimen not shown: postnatal day 10 (more

)

Structure	Level	Pattern	Image	Note
TS28: femur	Present

Specimen not shown: postnatal day 10; Fgfr2^tm1Dor/Fgfr2^tm1.1Dor, Twist2^tm1(cre)Dor/Twist2⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS28: femur	Present			Similar as in the control.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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