Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:171050
Thiagarajan RD, et al., Identification of anchor genes during kidney development defines ontological relationships, molecular subcompartments and regulatory pathways. PLoS One. 2011;6(2):e17286
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:7840422
|
|
Gene symbol:
|
Sord
|
|
Gene name:
|
sorbitol dehydrogenase
|
|
Probe:
|
RIKEN clone 4930403C20 subclone
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
Assay notes:
|
Authors described this gene as an 'anchor gene', which was defined as "genes whose expression is absolutely specific to a given anatomical compartment and/or developmental stage" in mouse kidney development.
|
|
|
Results
|
Specimen
5B':
embryonic day 15.5
(more  )
Specimen
5B':
(close  )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cortical early proximal tubule |
Present |
|
5B' Sord
|
Section in situ hybridization confirmed specific expression in early proximal tubule. Validated anchor gene. Expression was restricted to stage IV tubules.
|
Specimen
not shown:
postnatal adult
(more )
Note: Data from diagram in figure 5B.
Specimen
not shown:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Data from diagram in figure 5B.
|
|
Analysis Tools
