Shox2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:355648 Abassah-Oppong S, et al., A gene desert required for regulatory control of pleiotropic Shox2 expression and embryonic survival. Nat Commun. 2024 Oct 10;15(1):8793
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7834405
Gene symbol:	Shox2
Gene name:	SHOX homeobox 2

Antibody:	anti-SHOX2 (JK-6E): sc-81955
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

4C WT Merge ©

4C WT SHOX2 ©

Specimen 4C WT: embryonic day 11.5 (more

)
Note: Triple labeled: green - Shox2; red - Hcn4 (assay MGI:7834450); blue - Nkx2-5 (assay MGI:7834453).

Structure	Level	Pattern	Image	Note
TS19: sinoatrial node	Present		4C WT merge boxed , 4C WT SHOX2 , 4C WT Merge
TS19: sinus venosus	Present		4C WT merge boxed , 4C WT SHOX2 , 4C WT Merge

Specimen 4C GD^delta/delta: embryonic day 11.5; Del(3Rr805-Rr776)2Lap/Del(3Rr805-Rr776)2Lap (more

)
Note: Triple labeled: green - Shox2; red - Hcn4 (assay MGI:7834450); blue - Nkx2-5 (assay MGI:7834453).

Structure	Level	Pattern	Image	Note
TS19: sinoatrial node	Absent		4C GD^delta/delta Merge , 4C GD^delta/delta merge boxed , 4C GD^delta/delta SHOX2
TS19: sinus venosus	Absent		4C GD^delta/delta Merge , 4C GD^delta/delta merge boxed , 4C GD^delta/delta SHOX2

S4D WT ©

Specimen S4D WT: embryonic day 11.5 (more

)
Note: Double labeled: green - Shox2; red - Acta2 (assay MGI:7834463).

Structure	Level	Pattern	Image	Note
TS19: cardiac muscle tissue	Present		S4D WT
TS19: sinus venosus	Present		S4D WT

Specimen S4D GD^delta/delta: embryonic day 11.5; Del(3Rr805-Rr776)2Lap/Del(3Rr805-Rr776)2Lap (more

)
Note: Double labeled: green - Shox2; red - Acta2 (assay MGI:7834463).

Structure	Level	Pattern	Image	Note
TS19: cardiac muscle tissue	Absent		S4D GD^delta/delta
TS19: sinus venosus	Absent		S4D GD^delta/delta

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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