About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:174095 Cai Z, et al., Loss of shp2-mediated mitogen-activated protein kinase signaling in muller glial cells results in retinal degeneration. Mol Cell Biol. 2011 Jul;31(14):2973-83
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7788479
Gene symbol: Mapk1
Gene name: mitogen-activated protein kinase 1
Antibody: anti-phospho-ERK1/2 (D13.14.4E; #4370)
Assay notes: The antibody used recognizes the phosphorylated form of the protein. The specificity of the antibody used was not detailed; both family members have been annotated. A tyramide-enhanced immunofluorescence technique was used.
Results
Specimen 3E/F/H WT: postnatal day 21 (more )
Note: Double labeled: red - Mapk1 and Mapk3; green - Glul (assay MGI:7788465).
Structure Cell Type Level Pattern Image Note
TS28: retina inner nuclear layer Mueller cell Weak 3F WT , 3E WT , 3H WT Coexpressed with glutamine synthetase in the cell bodies.
TS28: retina ganglion cell layer Weak 3E WT
Specimen 3E/F/H cKO: postnatal day 21; Ptpn11tm1Gsf/Ptpn11tm1Gsf, Tg(Six3-cre)69Frty/0 (more )
Note: Conditional mutant. Double labeled: red - Mapk1 and Mapk3; green - Glul (assay MGI:7788465).
Structure Cell Type Level Pattern Image Note
TS28: retina inner nuclear layer Mueller cell Absent 3F cKO , 3E cKO , 3H cKO
TS28: retina ganglion cell layer Weak 3E cKO
Specimen 6K: postnatal day 21 (more )
Structure Cell Type Level Pattern Image Note
TS28: retina inner nuclear layer Mueller cell Weak 6K
Specimen 6L: postnatal day 21; Ptpn11tm1Gsf/Ptpn11tm1Gsf, Tg(Six3-cre)69Frty/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: retina inner nuclear layer Mueller cell Absent 6L
Specimen 6M: postnatal day 21; Krastm4Tyj/Krastm4Tyj, Ptpn11tm1Gsf/Ptpn11tm1Gsf, Tg(Six3-cre)69Frty/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: retina inner nuclear layer Mueller cell Weak 6M Expression was restored in the Muller glial cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
09/30/2025
MGI 6.24 		The Jackson Laboratory