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Gene Expression Data
Assay Details
Assay
Reference: J:298691 Wanigasuriya I, et al., Smchd1 is a maternal effect gene required for genomic imprinting. Elife. 2020 Nov 13;9:e55529
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7785346
Gene symbol: Smchd1
Gene name: SMC hinge domain containing 1
Reporter: GFP
Direct Detection
Results
Specimen 1c Smchd1GFP/GFP F x Smchd1GFP/GFP M E1.5: embryonic day 1.5; Smchd1tm1.1Mebl/Smchd1tm1.1Mebl (more )
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present 1c E1.5 top
Specimen 1c Smchd1+/+ F x Smchd1GFP/GFP M E1.5: embryonic day 1.5; Smchd1tm1.1Mebl/Smchd1+ (more )
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Absent 1c E1.5 bottom Paternal Smchd1-GFP was not detected at this stage.
Specimen 1c Smchd1GFP/GFP F x Smchd1GFP/GFP M E2.25: embryonic day 2.25; Smchd1tm1.1Mebl/Smchd1tm1.1Mebl (more )
Structure Level Pattern Image Note
TS3: 4-8 cell stage embryo Present 1c E2.25 top
Specimen 1c Smchd1+/+ F x Smchd1GFP/GFP M E2.25: embryonic day 2.25; Smchd1tm1.1Mebl/Smchd1+ (more )
Structure Level Pattern Image Note
TS3: 4-8 cell stage embryo Absent 1c E2.25 bottom Paternal Smchd1-GFP was not detected at this stage.
Specimen 1c Smchd1GFP/GFP F x Smchd1GFP/GFP M E2.5: embryonic day 2.5; Smchd1tm1.1Mebl/Smchd1tm1.1Mebl (more )
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present 1c E2.5 top
Specimen 1c Smchd1+/+ F x Smchd1GFP/GFP M E2.5: embryonic day 2.5; Smchd1tm1.1Mebl/Smchd1+ (more )
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Absent 1c E2.5 bottom Paternal Smchd1-GFP was not detected at this stage.
Specimen 1c Smchd1GFP/GFP F x Smchd1GFP/GFP M E2.75: embryonic day 2.75; Smchd1tm1.1Mebl/Smchd1tm1.1Mebl (more )
Structure Level Pattern Image Note
TS4: compacted morula Present 1c E2.75 top
Specimen 1c Smchd1+/+ F x Smchd1GFP/GFP M E2.75: embryonic day 2.75; Smchd1tm1.1Mebl/Smchd1+ (more )
Structure Level Pattern Image Note
TS4: compacted morula Present 1c E2.75 bottom Paternal Smchd1-GFP was observed in 16-cell embryo (33% positive).
Specimen 1c Smchd1GFP/GFP F x Smchd1GFP/GFP M E3.5: embryonic day 3.5; Smchd1tm1.1Mebl/Smchd1tm1.1Mebl (more )
Structure Level Pattern Image Note
TS5: blastocyst Present 1c E3.5 top
Specimen 1c Smchd1+/+ F x Smchd1GFP/GFP M E3.5: embryonic day 3.5; Smchd1tm1.1Mebl/Smchd1+ (more )
Structure Level Pattern Image Note
TS5: blastocyst Present 1c E3.5 bottom Paternal Smchd1-GFP was observed in 16-cell embryo (100% positive).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory