Pecam1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:173838 Manuylov NL, et al., Conditional ablation of Gata4 and Fog2 genes in mice reveals their distinct roles in mammalian sexual differentiation. Dev Biol. 2011 May 15;353(2):229-41
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7782572
Gene symbol:	Pecam1
Gene name:	platelet/endothelial cell adhesion molecule 1

Antibody:	Anti-PECAM1
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

2A Control ©

Specimen 2A control: embryonic day 11.5 (more

)
Note: Double labeled: green - Pecam1; red - Gata4 (assay MGI:7782571).

Structure	Level	Pattern	Image	Note
TS19: developing vasculature of reproductive system	Present		2A Control

2A cKO ©

Specimen 2A GATA4cKO: embryonic day 11.5; Gata4^tm1.1Wtp/Gata4^tm1.1Wtp, Tg(Nr5a1-cre)2Klp/0 (more

)
Note: Conditional mutant. Double labeled: green - Pecam1; red - Gata4 (assay MGI:7782571).

Structure	Level	Pattern	Image	Note
TS19: developing vasculature of reproductive system	Present		2A cKO

2B Control ©

Specimen 2B control: embryonic day 12.5 (more

)
Note: Double labeled: green - Pecam1; red - Gata4 (assay MGI:7782571).

Structure	Level	Pattern	Image	Note
TS20: developing vasculature of testis	Present		2B Control
TS20: germ cell of testis	Present		2B Control

2B cKO ©

Specimen 2B GATA4cKO: embryonic day 12.5; Gata4^tm1.1Wtp/Gata4^tm1.1Wtp, Tg(Nr5a1-cre)2Klp/0 (more

)
Note: Conditional mutant. Double labeled: green - Pecam1; red - Gata4 (assay MGI:7782571).

Structure	Level	Pattern	Image	Note
TS20: developing vasculature of testis	Present		2B cKO

2C Control ©

Specimen 2C control: embryonic day 11.5 (more

)
Note: Double labeled: green - Pecam1; red - Zfpm2 (assay MGI:7782573).

Structure	Level	Pattern	Image	Note
TS19: developing vasculature of reproductive system	Present		2C Control

2C cKO ©

Specimen 2C FOG2cKO: embryonic day 11.5; Tg(Nr5a1-cre)2Klp/0, Zfpm2^tm2Sho/Zfpm2^tm2Sho (more

)
Note: Conditional mutant. Double labeled: green - Pecam1; red - Zfpm2 (assay MGI:7782573).

Structure	Level	Pattern	Image	Note
TS19: developing vasculature of reproductive system	Present		2C cKO

2D Control ©

Specimen 2D control: embryonic day 13.5 (more

)
Note: Double labeled: green - Pecam1; red - Zfpm2 (assay MGI:7782573).

Structure	Level	Pattern	Image	Note
TS21: developing vasculature of testis	Present		2D Control
TS21: germ cell of testis	Present		2D Control

2D cKO ©

Specimen 2D FOG2cKO: embryonic day 13.5; Tg(Nr5a1-cre)2Klp/0, Zfpm2^tm2Sho/Zfpm2^tm2Sho (more

)
Note: Conditional mutant. Double labeled: green - Pecam1; red - Zfpm2 (assay MGI:7782573).

Structure	Level	Pattern	Image	Note
TS21: developing vasculature of testis	Present		2D cKO
TS21: germ cell of testis	Present		2D cKO

3C Control ©

Specimen 3C control: embryonic day 14.5 (more

)
Note: Double labeled: green - Pecam1; red - Pou5f1 (assay MGI:7783653).

Structure	Level	Pattern	Image	Note
TS22: developing vasculature of testis	Present		3C Control

3C cKO ©

Specimen 3C GATA4cKO: embryonic day 14.5; Gata4^tm1.1Wtp/Gata4^tm1.1Wtp, Tg(Nr5a1-cre)2Klp/0 (more

)
Note: Conditional mutant. Double labeled: green - Pecam1; red - Pou5f1 (assay MGI:7783653).

Structure	Level	Pattern	Image	Note
TS22: developing vasculature of testis	Present		3C cKO	Similar as in the control.

4B ©

Specimen 4B control: embryonic day 18.5 (more

)
Note: Double labeled: green - Pecam1; red - Sox9 (assay MGI:7783652).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: Sertoli cell	Sertoli cell	Present		4B

4E ©

Specimen 4E GATA4cKO: embryonic day 18.5; Gata4^tm1.1Wtp/Gata4^tm1.1Wtp, Tg(Nr5a1-cre)2Klp/0 (more

)
Note: Conditional mutant. Double labeled: green - Pecam1; red - Sox9 (assay MGI:7783652).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: Sertoli cell	Sertoli cell	Present		4E	Normal expression, but testis cords in the mutant appeared highly irregular.

5A left ©

5A right ©

Specimen 5A control: embryonic day 13.5 (more

)
Note: Double labeled: green - Pecam1; red - Dmrt1 (assay MGI:7783713).

Structure	Level	Pattern	Image	Note
TS21: germ cell of testis	Present		5A left , 5A right

5B left ©

5B right ©

Specimen 5B GATA4cKO: embryonic day 13.5; Gata4^tm1.1Wtp/Gata4^tm1.1Wtp, Tg(Nr5a1-cre)2Klp/0 (more

)
Note: Conditional mutant. Double labeled: green - Pecam1; red - Dmrt1 (assay MGI:7783713).

Structure	Level	Pattern	Image	Note
TS21: germ cell of testis	Present		5B left , 5B right

6A ©

Specimen 6A control: embryonic day 13.5 (more

)
Note: Double labeled: green - Pecam1; red - Dmrt1 (assay MGI:7783713).

Structure	Level	Pattern	Image	Note
TS21: developing vasculature of testis	Present		6A
TS21: germ cell of testis	Present		6A

6B ©

Specimen 6B FOG2cKO: embryonic day 13.5; Tg(Nr5a1-cre)2Klp/0, Zfpm2^tm2Sho/Zfpm2^tm2Sho (more

)
Note: Conditional mutant. Double labeled: green - Pecam1; red - Dmrt1 (assay MGI:7783713).

Structure	Level	Pattern	Image	Note
TS21: developing vasculature of testis	Present		6B
TS21: germ cell of testis	Present		6B

6C ©

Specimen 6C FOG2null: embryonic day 13.5; Zfpm2^tm1Sho/Zfpm2^tm1Sho (more

)
Note: Double labeled: green - Pecam1; red - Dmrt1 (assay MGI:7783713).

Structure	Level	Pattern	Image	Note
TS21: developing vasculature of testis	Present		6C
TS21: germ cell of testis	Present		6C

9A ©

Specimen 9A control: embryonic day 14.5 (more

)
Note: Double labeled: green - Pecam1; red - Sox9 (assay MGI:7783652).

Structure	Level	Pattern	Image	Note
TS22: developing vasculature of testis	Present		9A
TS22: germ cell of testis	Present		9A

9B ©

Specimen 9B FOG2cKO: embryonic day 14.5; Tg(Nr5a1-cre)2Klp/0, Zfpm2^tm2Sho/Zfpm2^tm2Sho (more

)
Note: Conditional mutant. Hermaphrodite. Double labeled: green - Pecam1; red - Sox9 (assay MGI:7783652).

Structure	Level	Pattern	Image	Note
TS22: developing vasculature of reproductive system	Present		9B
TS22: germ cell of gonad	Present		9B

10A ©

Specimen 10A XX control: embryonic day 14.5 (more

)
Note: Double labeled: green - Pecam1; red - Foxl2 (assay MGI:7783741).

Structure	Level	Pattern	Image	Note
TS22: developing vasculature of ovary	Present		10A
TS22: germ cell of ovary	Present		10A

10B ©

Specimen 10B XY control: embryonic day 14.5 (more

)
Note: Double labeled: green - Pecam1; red - Foxl2 (assay MGI:7783741).

Structure	Level	Pattern	Image	Note
TS22: developing vasculature of testis	Present		10B
TS22: germ cell of testis	Present		10B

10C ©

Specimen 10C XY FOG2cKO: embryonic day 14.5; Tg(Nr5a1-cre)2Klp/0, Zfpm2^tm2Sho/Zfpm2^tm2Sho (more

)
Note: Conditional mutant. Hermaphrodite. Double labeled: green - Pecam1; red - Foxl2 (assay MGI:7783741).

Structure	Level	Pattern	Image	Note
TS22: developing vasculature of reproductive system	Present		10C
TS22: germ cell of gonad	Present		10C

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