Pou5f1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:173838 Manuylov NL, et al., Conditional ablation of Gata4 and Fog2 genes in mice reveals their distinct roles in mammalian sexual differentiation. Dev Biol. 2011 May 15;353(2):229-41
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7783653
Gene symbol:	Pou5f1
Gene name:	POU domain, class 5, transcription factor 1

Antibody:	anti-OCT4
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

3C Control ©

Specimen 3C control: embryonic day 14.5 (more

)
Note: Double labeled: red - Pou5f1; green - Pecam1 (assay MGI:7782572).

Structure	Level	Pattern	Image	Note
TS22: germ cell of testis	Present		3C Control

3C cKO ©

Specimen 3C GATA4cKO: embryonic day 14.5; Gata4^tm1.1Wtp/Gata4^tm1.1Wtp, Tg(Nr5a1-cre)2Klp/0 (more

)
Note: Conditional mutant. Double labeled: red - Pou5f1; green - Pecam1 (assay MGI:7782572).

Structure	Level	Pattern	Image	Note
TS22: germ cell of testis	Present		3C cKO	Similar expression as in the control, although irregularity in cord formation was seen.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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