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Gene Expression Data
Assay Details
Assay
Reference: J:282348 Dasgupta K, et al., Molecular patterning of the embryonic cranial mesenchyme revealed by genome-wide transcriptional profiling. Dev Biol. 2019 Nov 15;455(2):434-448
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7781803
Gene symbol: Creb1
Gene name: cAMP responsive element binding protein 1
Antibody: anti-Phospho-CREB (Ser133) (87G3) Rabbit mAb #9198
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 8K: embryonic day 12.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant. Double labeled: red - Creb1; green - R26R-YFP.
Structure Level Pattern Image Note
TS20: early migrating mesenchyme Strong Widespread 8L , 8M , 8K
TS20: supraorbital mesenchyme Strong Regionally restricted 8N , 8K , 8L Expression was high in parts of the supraorbital mesenchyme (SOM).
Specimen 8O: embryonic day 13.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant. Double labeled: red - Creb1; green - R26R-YFP.
Structure Level Pattern Image Note
TS21: early migrating mesenchyme Strong 8O , 8Q , 8P
TS21: supraorbital mesenchyme Weak 8R , 8O , 8P
Specimen S12J: embryonic day 12.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant. Double labeled: red - Creb1; green - R26R-YFP.
Structure Level Pattern Image Note
TS20: early migrating mesenchyme Strong Widespread S12L , S12K , S12J
TS20: supraorbital mesenchyme Strong Regionally restricted S12K , S12M , S12J Expression was high in parts of the supraorbital mesenchyme (SOM).
Specimen S12N: embryonic day 13.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant. Double labeled: red - Creb1; green - R26R-YFP.
Structure Level Pattern Image Note
TS21: early migrating mesenchyme Strong S12O , S12N , S12P
TS21: supraorbital mesenchyme Present Regionally restricted S12Q , S12N , S12O The apical-basal difference was not as apparent at the parietal bone level at this stage.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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