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Gene Expression Data
Assay Details
Assay
Reference: J:173604 Allen BL, et al., Overlapping Roles and Collective Requirement for the Coreceptors GAS1, CDO, and BOC in SHH Pathway Function. Dev Cell. 2011 Jun 14;20(6):775-87
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7781596
Gene symbol: Nkx2-2
Gene name: NK2 homeobox 2
Antibody: 74.5A5
Detection system: Secondary antibody
Results
Specimen 2Q: embryonic day 10.5 (more )
Note: Double labeled: red - Nkx2-2; green - Olig2 (assay MGI:7781595).
Structure Level Pattern Image Note
TS17: neural tube lateral wall Present Regionally restricted 2Q Expressed in V3 interneuron progenitors in the ventral region.
Specimen 2R: embryonic day 10.5; Boctm2Rsk/Boctm2Rsk (more )
Note: Double labeled: red - Nkx2-2; green - Olig2 (assay MGI:7781595).
Structure Level Pattern Image Note
TS17: neural tube lateral wall Present Regionally restricted 2R Expressed in V3 interneuron progenitors in the ventral region, similar as in the wild-type. The increased expression of Foxa2 and Nkx2-2 double positive cells in Cdo-/- mutants was not seen in Boc-/-.
Specimen 2S: embryonic day 10.5; Cdontm1Rsk/Cdontm1Rsk (more )
Note: Double labeled: red - Nkx2-2; green - Olig2 (assay MGI:7781595).
Structure Level Pattern Image Note
TS17: neural tube floor plate Present 2S Increased expression of Foxa2 and Nkx2-2 double positive cells.
TS17: neural tube lateral wall Present Regionally restricted 2S Expressed in V3 interneuron progenitors in the ventral region.
Specimen 2T: embryonic day 10.5; Boctm2Rsk/Boctm2Rsk, Cdontm1Rsk/Cdon+ (more )
Note: Double labeled: red - Nkx2-2; green - Olig2 (assay MGI:7781595).
Structure Level Pattern Image Note
TS17: neural tube lateral wall Present Regionally restricted 2T Expressed in V3 interneuron progenitors in the ventral region, similar as in the wild-type.
Specimen 2U: embryonic day 10.5; Cdontm1Rsk/Cdontm1Rsk, Boctm2Rsk/Boctm2Rsk (more )
Note: Double labeled: red - Nkx2-2; green - Olig2 (assay MGI:7781595).
Structure Level Pattern Image Note
TS17: neural tube Present Regionally restricted 2U Reduced expression in the ventral region, but not as severe as the loss of Olig2+ cells.
Specimen 2V: embryonic day 10.5; Gli2tm1Alj/Gli2tm1Alj (more )
Note: Double labeled: red - Nkx2-2; green - Olig2 (assay MGI:7781595).
Structure Level Pattern Image Note
TS17: neural tube Absent 2V
Specimen 2W: embryonic day 10.5; Gas1tm2Fan/Gas1tm2Fan (more )
Note: Double labeled: red - Nkx2-2; green - Olig2 (assay MGI:7781595).
Structure Level Pattern Image Note
TS17: neural tube floor plate Present 2W Coexpression of Foxa2 and Nkx2-2 was seen.
Specimen 2X: embryonic day 10.5; Boctm2Rsk/Boctm2Rsk, Gas1tm2Fan/Gas1tm2Fan (more )
Note: Double labeled: red - Nkx2-2; green - Olig2 (assay MGI:7781595).
Structure Level Pattern Image Note
TS17: neural tube Absent 2X
Specimen 3G: embryonic day 9.5; Boctm2Rsk/Boctm2Rsk (more )
Note: Double labeled: red - Nkx2-2; green - Olig2 (assay MGI:7781595).
Structure Level Pattern Image Note
TS15: neural tube lateral wall Present Regionally restricted 3G Expressed in V3 interneuron progenitors in the ventral region, similar as in the wild-type.
Specimen 3H: embryonic day 9.5; Cdontm1Rsk/Cdontm1Rsk, Boctm2Rsk/Boctm2Rsk (more )
Note: Double labeled: red - Nkx2-2; green - Olig2 (assay MGI:7781595).
Structure Level Pattern Image Note
TS15: neural tube lateral wall Weak Regionally restricted 3H Expressed in a few positive V3 interneuron progenitors in the ventral region.
Specimen 3I: embryonic day 9.5; Gli2tm1Alj/Gli2tm1Alj (more )
Note: Double labeled: red - Nkx2-2; green - Olig2 (assay MGI:7781595).
Structure Level Pattern Image Note
TS15: neural tube lateral wall Absent 3I
Specimen 3N: embryonic day 9.5; Cdontm1Rsk/Cdontm1Rsk, Gas1tm2Fan/Gas1tm2Fan (more )
Note: Double labeled: red - Nkx2-2; green - Olig2 (assay MGI:7781595).
Structure Level Pattern Image Note
TS15: neural tube lateral wall Weak Regionally restricted 3N Expressed in a few positive V3 interneuron progenitors in the ventral region.
Specimen 3O: embryonic day 9.5; Boctm2Rsk/Boctm2Rsk, Gas1tm2Fan/Gas1tm2Fan (more )
Note: Double labeled: red - Nkx2-2; green - Olig2 (assay MGI:7781595).
Structure Level Pattern Image Note
TS15: neural tube lateral wall Weak Regionally restricted 3O Expressed in a few positive V3 interneuron progenitors in the ventral region.
Specimen 3S: embryonic day 12.5 (more )
Note: Double labeled: red - Nkx2-2; green - Olig2 (assay MGI:7781595).
Structure Level Pattern Image Note
TS20: spinal cord lateral wall Present Regionally restricted 3S Expressed in V3 interneuron progenitors in the ventral region.
Specimen 3T: embryonic day 12.5; Gas1tm2Fan/Gas1tm2Fan (more )
Note: Double labeled: red - Nkx2-2; green - Olig2 (assay MGI:7781595).
Structure Level Pattern Image Note
TS20: spinal cord lateral wall Present Regionally restricted 3T Expression in V3 interneuron progenitors in the ventral region was maintained.
Specimen 3U: embryonic day 12.5; Gli2tm1Alj/Gli2tm1Alj (more )
Note: Double labeled: red - Nkx2-2; green - Olig2 (assay MGI:7781595).
Structure Level Pattern Image Note
TS20: spinal cord lateral wall Absent 3U
Specimen 7I: embryonic day 9.5; Boctm2Rsk/Boctm2Rsk, Cdontm1Rsk/Cdon+, Gas1tm2Fan/Gas1+ (more )
Note: Double labeled: red - Nkx2-2; green - Olig2 (assay MGI:7781595).
Structure Level Pattern Image Note
TS15: neural tube lateral wall Present Regionally restricted 7I Expressed in V3 interneuron progenitors in the ventral region.
Specimen 7J: embryonic day 9.5; Boctm2Rsk/Boctm2Rsk, Cdontm1Rsk/Cdon+, Gas1tm2Fan/Gas1tm2Fan (more )
Note: Double labeled: red - Nkx2-2; green - Olig2 (assay MGI:7781595).
Structure Level Pattern Image Note
TS15: neural tube lateral wall Trace Regionally restricted 7J Expressed in a few positive V3 interneuron progenitors in the ventral region.
Specimen 7K: embryonic day 9.5; Boctm2Rsk/Boctm2Rsk, Cdontm1Rsk/Cdontm1Rsk, Gas1tm2Fan/Gas1tm2Fan (more )
Note: Double labeled: red - Nkx2-2; green - Olig2 (assay MGI:7781595).
Structure Level Pattern Image Note
TS15: neural tube Absent 7K
Specimen 7L: embryonic day 9.5; Shhtm1Amc/Shhtm1Amc (more )
Note: Double labeled: red - Nkx2-2; green - Olig2 (assay MGI:7781595).
Structure Level Pattern Image Note
TS15: neural tube Absent 7L
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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