Antibody:	anti-Sox10
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Cell Type	Level	Pattern	Image	Note
TS17: cranial mesenchyme	migratory cranial neural crest cell	Present		3A WT
TS17: migrating neural crest	migratory cranial neural crest cell	Present		3A WT

Structure	Cell Type	Level	Pattern	Image	Note
TS17: cranial mesenchyme	migratory cranial neural crest cell	Present		3A mray/mray	The number of cranial positive migratory neural crest cell was significantly reduced (2.5-fold decrease) in anterior/midbrain positions.
TS17: migrating neural crest	migratory cranial neural crest cell	Present		3A mray/mray	The number of cranial positive migratory neural crest cell was significantly reduced (2.5-fold decrease) in anterior/midbrain positions.

Structure	Cell Type	Level	Pattern	Image	Note
TS17: migrating neural crest	migratory trunk neural crest cell	Present		3B WT
TS17: trunk mesenchyme derived from neural crest	migratory trunk neural crest cell	Present		3B WT

Structure	Cell Type	Level	Pattern	Image	Note
TS17: migrating neural crest	migratory trunk neural crest cell	Present		3B mray/mray	A lesser, although still statistically significant, decrease ( about 20%) was detected posteriorly for the truncal neural crest cell at the level of the hindlimb.
TS17: trunk mesenchyme derived from neural crest	migratory trunk neural crest cell	Present		3B mray/mray	A lesser, although still statistically significant, decrease ( about 20%) was detected posteriorly for the truncal neural crest cell at the level of the hindlimb.