Manf Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:142284 Lindholm P, et al., MANF is widely expressed in mammalian tissues and differently regulated after ischemic and epileptic insults in rodent brain. Mol Cell Neurosci. 2008 Nov;39(3):356-71
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7779866
Gene symbol:	Manf
Gene name:	mesencephalic astrocyte-derived neurotrophic factor

Antibody:	anti-Manf
Detection system:	Secondary antibody coupled to horseradish peroxidase

Results

Specimen 5A: postnatal adult (more

)

Structure	Level	Image	Note
TS28: cortical layer II	Present	5A	relatively strong
TS28: cortical layer III	Present	5A	relatively strong
TS28: cortical layer IV	Present	5A	relatively strong
TS28: cortical layer V	Present	5A	relatively strong
TS28: cortical layer VI	Present	5A	relatively strong

Specimen 5B: postnatal adult (more

)

Structure	Level	Image	Note
TS28: cortical layer II	Present	5B	relatively strong
TS28: cortical layer IV	Present	5B	relatively strong
TS28: cortical layer V	Present	5B	relatively strong
TS28: cortical layer VI	Present	5B	relatively strong
TS28: cortical layer III	Strong	5B	Pyramidal-like, large cells in the layer III had intense labelling.

Specimen 5E: postnatal adult (more

)

Structure	Level	Image
TS28: hippocampus CA1	Present	5E
TS28: hippocampus CA2	Present	5E
TS28: hippocampus CA3	Present	5E

Specimen 5F: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		5F

Specimen 5G: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Weak		5G

Specimen 5H: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: substantia nigra pars reticulata	Present		5H

Specimen 5I: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: substantia nigra pars reticulata	Present		5I

Specimen 5M: postnatal adult (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Strong		5M
TS28: cerebellum molecular layer		Present		5M	Expressed in Purkinje cell dendrites extending to the molecular layer.

Specimen 5N: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: spinal cord	Strong		5N

Specimen 5O: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: choroid plexus	Strong		5O	Expressed in non-neuronal cells.

Specimen 5P: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: spinal cord	Strong		5P

Specimen 5Q: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: salivary gland	Strong		5Q	Expressed in serous tubules.

Specimen 5S: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: primary spermatocyte	Present		5S	early spermatocytes

Specimen 6A: postnatal day 1 (more

)

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb mitral cell layer	Strong		6A

Specimen 6B: postnatal day 1 (more

)

Structure	Level	Pattern	Image	Note
TS27: corpus striatum	Present		6B

Specimen 6C: postnatal day 1 (more

)

Structure	Level	Pattern	Image	Note
TS27: hippocampus	Present		6C

Specimen 6D: postnatal day 1 (more

)

Structure	Level	Pattern	Image	Note
TS27: hippocampus	Present		6D

Specimen 6E: postnatal day 1 (more

)

Structure	Level	Pattern	Image	Note
TS27: thalamus	Present		6E

Specimen 6F: postnatal day 1 (more

)

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present		6F

Specimen 6G: postnatal day 1 (more

)

Structure	Level	Pattern	Image	Note
TS27: substantia nigra	Present		6G

Specimen 6H: postnatal day 10 (more

)

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		6H

Specimen 6I: postnatal day 10 (more

)

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA3	Present		6I	relatively strong

Specimen 6J: postnatal day 10 (more

)

Structure	Level	Pattern	Image	Note
TS28: thalamus	Present		6J	relatively strong

Specimen 6K: postnatal day 10 (more

)

Structure	Level	Pattern	Image	Note
TS28: caudate-putamen	Weak		6K

Specimen 6L: postnatal day 10 (more

)

Structure	Level	Pattern	Image	Note
TS28: substantia nigra pars reticulata	Present		6L

Specimen 8A: embryonic day 14.0 (more

)

Structure	Level	Pattern	Image	Note
TS22: neocortex	Present	Regionally restricted	8A	Relatively high level expression in the roof.

Specimen 8B: embryonic day 14.0 (more

)

Structure	Level	Pattern	Image	Note
TS22: ganglionic eminence	Weak		8B

Specimen 8C: embryonic day 14.0 (more

)

Structure	Level	Pattern	Image	Note
TS22: median sulcus	Strong		8C

Specimen 8D: embryonic day 14.0 (more

)

Structure	Level	Pattern	Image	Note
TS22: choroid plexus	Strong		8D

Specimen 8E: embryonic day 16.0 (more

)

Structure	Level	Pattern	Image	Note
TS24: midbrain	Moderate	Regionally restricted	8E	Expressed in the developing substantia nigra.

Specimen 8F: embryonic day 16.0 (more

)

Structure	Level	Pattern	Image	Note
TS24: midbrain	Moderate	Regionally restricted	8F	Expressed in the developing substantia nigra.

Specimen 8H: embryonic day 17.0 (more

)

Structure	Level	Pattern	Image	Note
TS25: dorsal root ganglion	Present		8H

Specimen 8I: embryonic day 17.0 (more

)

Structure	Level	Pattern	Image	Note
TS25: trigeminal ganglion	Present		8I

Specimen 8J: embryonic day 17.0 (more

)

Structure	Level	Pattern	Image	Note
TS25: superior cervical ganglion	Present		8J

Specimen 8K: embryonic day 17.0 (more

)

Structure	Level	Pattern	Image	Note
TS25: spinal cord mantle layer	Present		8K	Relatively low expression, although some strongly positive cells were also found.

Specimen 8L: embryonic day 17.0 (more

)

Structure	Level	Pattern	Image	Note
TS25: submandibular gland	Strong		8L

Specimen 8M: embryonic day 17.0 (more

)

Structure	Level	Pattern	Image	Note
TS25: gland	Strong	Regionally restricted	8M	Expressed in serous tubules located in the nasal cavity.

Specimen 8N: embryonic day 17.0 (more

)

Structure	Level	Pattern	Image	Note
TS25: pancreas	Strong		8N

Specimen 8O: embryonic day 17.0 (more

)

Structure	Level	Pattern	Image	Note
TS25: vomeronasal organ	Strong		8O
TS25: olfactory epithelium	Strong	Single cells	8O	Expressed in several epithelial cells, including the olfactory epithelium.

Specimen not shown: embryonic day 13.0 (more

)

Structure	Level	Pattern	Image	Note
TS21: liver	Present
TS21: vertebral cartilage condensation	Present

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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