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Gene Expression Data
Assay Details
Assay
Reference: J:345594 Ming Z, et al., A role for TRPC3 in mammalian testis development. Front Cell Dev Biol. 2024;12:1337714
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7778354
Gene symbol: Trpc3
Gene name: transient receptor potential cation channel, subfamily C, member 3
Antibody: Anti-TRPC3 ab51560
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1C E11.5: embryonic day 11.5 (more )
Note: Double labeled: green - Trpc3; red - Gata4 (assay MGI:7778353).
Structure Level Pattern Image Note
TS20: testis Present Regionally restricted 1C E11.5 Trpc3/Gata4 , 1C E11.5 Trpc3 Expression was localized in somatic cells.
Specimen 1C E12.5: embryonic day 12.5 (more )
Note: Double labeled: green - Trpc3; red - Gata4 (assay MGI:7778353).
Structure Level Pattern Image Note
TS20: testis Present Regionally restricted 1C E12.5 Trpc3 , 1C E12.5 Trpc3/Gata4 Expression was localized in somatic cells.
Specimen 1C E13.5: embryonic day 13.5 (more )
Note: Double labeled: green - Trpc3; red - Gata4 (assay MGI:7778353).
Structure Cell Type Level Pattern Image Note
TS21: testis Sertoli cell Present 1C E13.5 Trpc3/Gata4 , 1C E13.5 Trpc3 Expression was detected in the Sertoli cell membrane and cytoplasm.
Specimen 1C E14.5: embryonic day 14.5 (more )
Note: Double labeled: green - Trpc3; red - Gata4 (assay MGI:7778353).
Structure Cell Type Level Pattern Image Note
TS22: testis Sertoli cell Present 1C E14.5 Trpc3 , 1C E14.5 Trpc3/Gata4 Expression was detected in the Sertoli cell membrane and cytoplasm.
TS22: testis interstitial cell Weak 1C E14.5 Trpc3/Gata4 , 1C E14.5 Trpc3
Specimen 1C E15.5: embryonic day 15.5 (more )
Note: Double labeled: green - Trpc3; red - Gata4 (assay MGI:7778353).
Structure Cell Type Level Pattern Image Note
TS23: testis Sertoli cell Present 1C E15.5 Trpc3 , 1C E15.5 Trpc3/Gata4 Expression was detected in the Sertoli cell membrane and cytoplasm.
TS23: testis interstitial cell Weak 1C E15.5 Trpc3/Gata4 , 1C E15.5 Trpc3
Specimen 1D E13.5: embryonic day 13.5 (more )
Note: Double labeled: green - Trpc3; red - Amh (assay MGI:7778357).
Structure Cell Type Level Pattern Image Note
TS21: testis Sertoli cell Present 1D E13.5 Trpc3/AMH , 1D E13.5 Trpc3 Expression was detected in the Sertoli cell membrane and cytoplasm, where it colocalized with Amh.
Specimen 1D E14.5: embryonic day 14.5 (more )
Note: Double labeled: green - Trpc3; red - Amh (assay MGI:7778357).
Structure Cell Type Level Pattern Image Note
TS22: testis Sertoli cell Present 1D E14.5 Trpc3/AMH , 1D E14.5 Trpc3 Expression was detected in the Sertoli cell membrane and cytoplasm, where it colocalized with Amh.
TS22: testis interstitial cell Weak 1D E14.5 Trpc3/AMH , 1D E14.5 Trpc3
Specimen 1D E15.5: embryonic day 15.5 (more )
Note: Double labeled: green - Trpc3; red - Amh (assay MGI:7778357).
Structure Cell Type Level Pattern Image Note
TS23: testis Sertoli cell Present 1D E15.5 Trpc3 , 1D E15.5 Trpc3/AMH Expression was detected in the Sertoli cell membrane and cytoplasm, where it colocalized with Amh.
TS23: testis interstitial cell Weak 1D E15.5 Trpc3 , 1D E15.5 Trpc3/AMH
Specimen S2 E11.5: embryonic day 11.5 (more )
Note: Double labeled: green - Trpc3; red - Pecam1 (assay MGI:7778363).
Structure Cell Type Level Pattern Image Note
TS20: coelomic vessel blood vessel endothelial cell Absent S2_E11.5 Trpc3 , S2_E11.5 Trpc3/Pecam1
Specimen S2 E12.5: embryonic day 12.5 (more )
Note: Double labeled: green - Trpc3; red - Pecam1 (assay MGI:7778363).
Structure Cell Type Level Pattern Image Note
TS20: coelomic vessel blood vessel endothelial cell Weak Regionally restricted S2_E12.5 Trpc3 , S2_E12.5 Trpc3/Pecam1 Weak co-localization with Pecam1 was observed in a subset of coelomic vessel endothelial cells.
Specimen S2 E13.5: embryonic day 13.5 (more )
Note: Double labeled: green - Trpc3; red - Pecam1 (assay MGI:7778363).
Structure Cell Type Level Pattern Image Note
TS21: coelomic vessel blood vessel endothelial cell Weak Regionally restricted S2_E13.5 Trpc3 , S2_E13.5 Trpc3/Pecam1 Weak co-localization with Pecam1 was observed in a subset of coelomic vessel endothelial cells.
Specimen S2 E14.5: embryonic day 14.5 (more )
Note: Double labeled: green - Trpc3; red - Pecam1 (assay MGI:7778363).
Structure Cell Type Level Pattern Image Note
TS22: coelomic vessel blood vessel endothelial cell Weak Regionally restricted S2_E14.5 Trpc3/Pecam1 , S2_E14.5 Trpc3 Weak co-localization with Pecam1 was observed in a subset of coelomic vessel endothelial cells.
TS22: testis Leydig cell Weak S2_E14.5 Trpc3 , S2_E14.5 Trpc3/Pecam1
Specimen S2 E15.5: embryonic day 15.5 (more )
Note: Double labeled: green - Trpc3; red - Pecam1 (assay MGI:7778363).
Structure Cell Type Level Pattern Image Note
TS23: coelomic vessel blood vessel endothelial cell Weak Regionally restricted S2_E15.5 Trpc3/Pecam1 , S2_E15.5 Trpc3 Weak co-localization with Pecam1 was observed in a subset of coelomic vessel endothelial cells.
TS23: testis Leydig cell Weak S2_E15.5 Trpc3 , S2_E15.5 Trpc3/Pecam1
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory