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Gene Expression Data
Assay Details
Assay
Reference: J:345594 Ming Z, et al., A role for TRPC3 in mammalian testis development. Front Cell Dev Biol. 2024;12:1337714
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7778353
Gene symbol: Gata4
Gene name: GATA binding protein 4
Antibody: anti-GATA-4 (sc-25310)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 1C E11.5: embryonic day 11.5 (more )
Note: Double labeled: red - Gata4; green - Trpc3 (assay MGI:7778354).
Structure Cell Type Level Pattern Image Note
TS20: testis Present Regionally restricted 1C E11.5 Gata4 , 1C E11.5 Trpc3/Gata4 Gata4 is a somatic cell nuclear marker.
TS20: testis Sertoli cell Strong 1C E11.5 Trpc3/Gata4 , 1C E11.5 Gata4 Strong expression was in the nucleus of Sertoli cells.
Specimen 1C E12.5: embryonic day 12.5 (more )
Note: Double labeled: red - Gata4; green - Trpc3 (assay MGI:7778354).
Structure Cell Type Level Pattern Image Note
TS20: testis Present Regionally restricted 1C E12.5 Trpc3/Gata4 , 1C E12.5 Gata4 Gata4 is a somatic cell nuclear marker.
TS20: testis Sertoli cell Strong 1C E12.5 Trpc3/Gata4 , 1C E12.5 Gata4 Strong expression was in the nucleus of Sertoli cells.
Specimen 1C E13.5: embryonic day 13.5 (more )
Note: Double labeled: red - Gata4; green - Trpc3 (assay MGI:7778354).
Structure Cell Type Level Pattern Image Note
TS21: testis Present Regionally restricted 1C E13.5 Gata4 , 1C E13.5 Trpc3/Gata4 Gata4 is a somatic cell nuclear marker.
TS21: testis Sertoli cell Strong 1C E13.5 Gata4 , 1C E13.5 Trpc3/Gata4 Strong expression was in the nucleus of Sertoli cells.
Specimen 1C E14.5: embryonic day 14.5 (more )
Note: Double labeled: red - Gata4; green - Trpc3 (assay MGI:7778354).
Structure Cell Type Level Pattern Image Note
TS22: testis Present Regionally restricted 1C E14.5 Gata4 , 1C E14.5 Trpc3/Gata4 Gata4 is a somatic cell nuclear marker.
TS22: testis Sertoli cell Strong 1C E14.5 Trpc3/Gata4 , 1C E14.5 Gata4 Strong expression was in the nucleus of Sertoli cells.
Specimen 1C E15.5: embryonic day 15.5 (more )
Note: Double labeled: red - Gata4; green - Trpc3 (assay MGI:7778354).
Structure Cell Type Level Pattern Image Note
TS23: testis Present Regionally restricted 1C E15.5 Gata4 , 1C E15.5 Trpc3/Gata4 Gata4 is a somatic cell nuclear marker.
TS23: testis Sertoli cell Strong 1C E15.5 Gata4 , 1C E15.5 Trpc3/Gata4 Strong expression was in the nucleus of Sertoli cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory