Gene Expression Data
Assay Details
Assay
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Reference:
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J:122300
Taoudi S, et al., Functional identification of the hematopoietic stem cell niche in the ventral domain of the embryonic dorsal aorta. Proc Natl Acad Sci U S A. 2007 May 29;104(22):9399-403
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7767923
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Gene symbol:
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Ptprc
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Gene name:
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protein tyrosine phosphatase receptor type C
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Antibody:
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anti-CD45
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Detection system:
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alkaline phosphatase
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Results
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Specimen
3B/C/E:
embryonic day 11.5
(more )
Note: Double labeled: green - Ptprc; red - Pecam1 (assay MGI:7767922).
Specimen
3B/C/E:
(close )
Genetic Background:
|
(C57BL/6 x CBA)F1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Acetone
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Ptprc; red - Pecam1 (assay MGI:7767922).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS19: blood |
AGM hematopoietic stem cell |
Present |
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3C
,
3B
,
3E
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Coexpressed with Pecam1 in cells localized predominantly to the luminal surface of the endothelial lining of the dorsal aorta, and usually incorporated within intraaortic clusters with occasional integration into the endothelial layer.
|
Specimen
4A/C:
embryonic day 11.5
(more )
Note: Luminal view. Double labeled: green - Ptprc; red - Pecam1 (assay MGI:7767922).
Specimen
4A/C:
(close )
Genetic Background:
|
(C57BL/6 x CBA)F1
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Age:
|
embryonic day 11.5
|
Sex:
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Not Specified
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Type:
|
whole mount
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Note:
|
Luminal view. Double labeled: green - Ptprc; red - Pecam1 (assay MGI:7767922).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS19: blood |
AGM hematopoietic stem cell |
Present |
|
4C
,
4A
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Coexpressed with Pecam1 in cells scattered on the floor but not in subendothelial mesenchymal layers of the dorsal aorta.
|
Specimen
4B:
embryonic day 11.5
(more )
Note: Subluminal view. Double labeled: green - Ptprc; red - Pecam1 (assay MGI:7767922).
Specimen
4B:
(close )
Genetic Background:
|
(C57BL/6 x CBA)F1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Note:
|
Subluminal view. Double labeled: green - Ptprc; red - Pecam1 (assay MGI:7767922).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS19: blood |
AGM hematopoietic stem cell |
Present |
|
4B
|
Coexpressed with Pecam1 in cells scattered on the floor but not in subendothelial mesenchymal layers of the dorsal aorta.
|
Specimen
5G:
embryonic day 11.5
(more )
Note: Double labeled: green - Ptprc; red - Pecam1 (assay MGI:7767922).
Specimen
5G:
(close )
Genetic Background:
|
(C57BL/6 x CBA)F1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
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Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Ptprc; red - Pecam1 (assay MGI:7767922).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS19: blood |
AGM hematopoietic stem cell |
Present |
|
5G
|
Expressed in the dorsal and ventral domains of the dorsal aorta.
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