Gene Expression Data
Assay Details
Assay
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Reference:
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J:122300
Taoudi S, et al., Functional identification of the hematopoietic stem cell niche in the ventral domain of the embryonic dorsal aorta. Proc Natl Acad Sci U S A. 2007 May 29;104(22):9399-403
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7767922
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Gene symbol:
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Pecam1
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Gene name:
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platelet/endothelial cell adhesion molecule 1
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Antibody:
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MEC 13.3
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Detection system:
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phycoerythrin
|
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Results
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Specimen
3B/D/E:
embryonic day 11.5
(more )
Note: Double labeled: red - Pecam1; green - Ptprc (assay MGI:7767923).
Specimen
3B/D/E:
(close )
Genetic Background:
|
(C57BL/6 x CBA)F1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Acetone
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pecam1; green - Ptprc (assay MGI:7767923).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS19: dorsal aorta endothelium |
aortic endothelial cell |
Present |
|
3E
,
3B
,
3D
|
|
TS19: blood |
AGM hematopoietic stem cell |
Present |
|
3B
,
3D
,
3E
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Coexpressed with CD45/Ptprc in cells localized predominantly to the luminal surface of the endothelial lining of the dorsal aorta, and usually incorporated within intraaortic clusters with occasional integration into the endothelial layer.
|
Specimen
4A/C:
embryonic day 11.5
(more )
Note: Luminal view. Double labeled: red - Pecam1; green - Ptprc (assay MGI:7767923).
Specimen
4A/C:
(close )
Genetic Background:
|
(C57BL/6 x CBA)F1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
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Note:
|
Luminal view. Double labeled: red - Pecam1; green - Ptprc (assay MGI:7767923).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS19: dorsal aorta endothelium |
aortic endothelial cell |
Present |
|
4A
,
4C
|
|
TS19: blood |
AGM hematopoietic stem cell |
Present |
|
4C
,
4A
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Coexpressed with CD45/Ptprc in cells scattered on the floor but not in subendothelial mesenchymal layers of the dorsal aorta.
|
Specimen
4B:
embryonic day 11.5
(more )
Note: Subluminal view. Double labeled: red - Pecam1; green - Ptprc (assay MGI:7767923).
Specimen
4B:
(close )
Genetic Background:
|
(C57BL/6 x CBA)F1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Note:
|
Subluminal view. Double labeled: red - Pecam1; green - Ptprc (assay MGI:7767923).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS19: dorsal aorta endothelium |
aortic endothelial cell |
Present |
|
4B
|
|
TS19: blood |
AGM hematopoietic stem cell |
Present |
|
4B
|
Coexpressed with CD45/Ptprc in cells scattered on the floor but not in subendothelial mesenchymal layers of the dorsal aorta.
|
Specimen
5E/F:
embryonic day 11.5
(more )
Note: dorsal domain of the dorsal aorta
Specimen
5E/F:
(close )
Genetic Background:
|
(C57BL/6 x CBA)F1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
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Note:
|
dorsal domain of the dorsal aorta
|
Specimen
5E'/F':
embryonic day 11.5
(more )
Note: ventral domain of the dorsal aorta
Specimen
5E'/F':
(close )
Genetic Background:
|
(C57BL/6 x CBA)F1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
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Note:
|
ventral domain of the dorsal aorta
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Specimen
5G:
embryonic day 11.5
(more )
Note: Double labeled: red - Pecam1; green - Ptprc (assay MGI:7767923).
Specimen
5G:
(close )
Genetic Background:
|
(C57BL/6 x CBA)F1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Pecam1; green - Ptprc (assay MGI:7767923).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS19: dorsal aorta endothelium |
aortic endothelial cell |
Present |
|
5G
|
Successful separation of dorsal and ventral domains were shown.
|
|