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Gene Expression Data
Assay Details
Assay
Reference: J:122300 Taoudi S, et al., Functional identification of the hematopoietic stem cell niche in the ventral domain of the embryonic dorsal aorta. Proc Natl Acad Sci U S A. 2007 May 29;104(22):9399-403
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7767922
Gene symbol: Pecam1
Gene name: platelet/endothelial cell adhesion molecule 1
Antibody: MEC 13.3
Detection system: phycoerythrin
Results
Specimen 3B/D/E: embryonic day 11.5 (more )
Note: Double labeled: red - Pecam1; green - Ptprc (assay MGI:7767923).
Structure Cell Type Level Pattern Image Note
TS19: dorsal aorta endothelium aortic endothelial cell Present 3E , 3B , 3D
TS19: blood AGM hematopoietic stem cell Present 3B , 3D , 3E Coexpressed with CD45/Ptprc in cells localized predominantly to the luminal surface of the endothelial lining of the dorsal aorta, and usually incorporated within intraaortic clusters with occasional integration into the endothelial layer.

Specimen 4A/C: embryonic day 11.5 (more )
Note: Luminal view. Double labeled: red - Pecam1; green - Ptprc (assay MGI:7767923).
Structure Cell Type Level Pattern Image Note
TS19: dorsal aorta endothelium aortic endothelial cell Present 4A , 4C
TS19: blood AGM hematopoietic stem cell Present 4C , 4A Coexpressed with CD45/Ptprc in cells scattered on the floor but not in subendothelial mesenchymal layers of the dorsal aorta.

Specimen 4B: embryonic day 11.5 (more )
Note: Subluminal view. Double labeled: red - Pecam1; green - Ptprc (assay MGI:7767923).
Structure Cell Type Level Pattern Image Note
TS19: dorsal aorta endothelium aortic endothelial cell Present 4B
TS19: blood AGM hematopoietic stem cell Present 4B Coexpressed with CD45/Ptprc in cells scattered on the floor but not in subendothelial mesenchymal layers of the dorsal aorta.

Specimen 5E/F: embryonic day 11.5 (more )
Note: dorsal domain of the dorsal aorta
Structure Cell Type Level Pattern Image Note
TS19: dorsal aorta endothelium aortic endothelial cell Present 5E , 5F dorsal domain of the dorsal aorta

Specimen 5E'/F': embryonic day 11.5 (more )
Note: ventral domain of the dorsal aorta
Structure Cell Type Level Pattern Image Note
TS19: dorsal aorta endothelium aortic endothelial cell Present 5E' , 5F'

Specimen 5G: embryonic day 11.5 (more )
Note: Double labeled: red - Pecam1; green - Ptprc (assay MGI:7767923).
Structure Cell Type Level Pattern Image Note
TS19: dorsal aorta endothelium aortic endothelial cell Present 5G Successful separation of dorsal and ventral domains were shown.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory