Eppin RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:171630 Bagheri-Fam S, et al., Defective survival of proliferating Sertoli cells and androgen receptor function in a mouse model of the ATR-X syndrome. Hum Mol Genet. 2011 Jun 1;20(11):2213-24
Assay type:	RT-PCR
MGI Accession ID:	MGI:7751470
Gene symbol:	Eppin
Gene name:	epididymal peptidase inhibitor

Probe:	Spinlw1-pF, Spinlw1-pR
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative RT-PCR. Relative expression was normalized to Sdha. The qRT-PCR data of ScAtrxKO testes were adjusted to account for the reduced Sertoli cell proportion relative to total cells.

Results

Image: 8_Spinlw1

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
P12 Control	P12	TS28: testis	Present	0.75 µg; total RNA	involves: 129P2/OlaHsd * C57BL/6 * SJL	Atrx^tm1Rjg/Y	Male
P12 cKO	P12	TS28: testis	Present (a)	0.75 µg; total RNA	involves: 129P2/OlaHsd * C57BL/6 * SJL	Atrx^tm1Rjg/Y, Plekha5^{Tg(AMH-cre)1Flor}/0	Male	Conditional mutant.
P50 Control	P50	TS28: testis	Present	0.75 µg; total RNA	involves: 129P2/OlaHsd * C57BL/6 * SJL	Atrx^tm1Rjg/Y	Male
P50 cKO	P50	TS28: testis	Present (b)	0.75 µg; total RNA	involves: 129P2/OlaHsd * C57BL/6 * SJL	Atrx^tm1Rjg/Y, Plekha5^{Tg(AMH-cre)1Flor}/0	Male	Conditional mutant.

Notes:
(a) Expression was significantly down-regulated by 2.5-fold compared with the control.
(b) Expression was down-regulated by 41%, although not significant, compared with the control.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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