Gene Expression Data
Assay Details
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Assay
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Reference:
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J:171630
Bagheri-Fam S, et al., Defective survival of proliferating Sertoli cells and androgen receptor function in a mouse model of the ATR-X syndrome. Hum Mol Genet. 2011 Jun 1;20(11):2213-24
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7751458
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Gene symbol:
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Amh
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Gene name:
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anti-Mullerian hormone
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Results
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Specimen
1A E14.5:
embryonic day 14.5
(more  )
Note: Double labeled: red - Amh; green - Atrx (assay MGI:7751460).
Specimen
1A E14.5:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Male
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Amh; green - Atrx (assay MGI:7751460).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: Sertoli cell |
Sertoli cell |
Present |
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1A E14.5
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Expressed in immature Sertoli cells.
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Specimen
1A E17.5:
embryonic day 17.5
(more )
Note: Double labeled: red - Amh; green - Atrx (assay MGI:7751460).
Specimen
1A E17.5:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 17.5
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Sex:
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Male
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Amh; green - Atrx (assay MGI:7751460).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS25: Sertoli cell |
Sertoli cell |
Present |
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1A E17.5
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Expressed in immature Sertoli cells.
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Specimen
1Bii:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * SJL
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Mutant Allele(s):
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Atrxtm1Rjg/Y
Plekha5Tg(AMH-cre)1Flor/0
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Age:
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embryonic day 14.5
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Sex:
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Male
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Conditional mutant. Double labeled: red - Amh; green - Atrx (assay MGI:7751460).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: Sertoli cell |
Sertoli cell |
Present |
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1Bii
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Expressed in immature Sertoli cells.
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Specimen
2B Control:
embryonic day 17.5; Atrxtm1Rjg/Y
(more )
Specimen
2B Control:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * SJL
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Mutant Allele(s):
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Atrxtm1Rjg/Y
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Age:
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embryonic day 17.5
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Sex:
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Male
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS25: Sertoli cell |
Sertoli cell |
Present |
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2B Control
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Expressed in immature Sertoli cells.
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Specimen
2B cKO:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * SJL
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Mutant Allele(s):
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Atrxtm1Rjg/Y
Plekha5Tg(AMH-cre)1Flor/0
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Age:
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embryonic day 17.5
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Sex:
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Male
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Conditional mutant.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS25: Sertoli cell |
Sertoli cell |
Present |
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2B cKO
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Fewer testis cords than control, particularly in the core region.
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Specimen
not shown:
embryonic day 15.5; Atrxtm1Rjg/Y
(more )
Note: Quantified in Fig 3A.
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * SJL
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Mutant Allele(s):
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Atrxtm1Rjg/Y
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Age:
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embryonic day 15.5
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Sex:
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Male
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Quantified in Fig 3A.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: Sertoli cell |
Sertoli cell |
Present |
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Expressed in immature Sertoli cells.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * SJL
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Mutant Allele(s):
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Atrxtm1Rjg/Y
Plekha5Tg(AMH-cre)1Flor/0
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Age:
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embryonic day 15.5
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Sex:
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Male
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Conditional mutant. Quantified in Fig 3A.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: Sertoli cell |
Sertoli cell |
Present |
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Modest reduction (about 20%) in the number of Sertoli cells when compared with the control.
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Specimen
not shown:
embryonic day 17.5; Atrxtm1Rjg/Y
(more )
Note: Quantified in Fig 3A.
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * SJL
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Mutant Allele(s):
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Atrxtm1Rjg/Y
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Age:
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embryonic day 17.5
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Sex:
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Male
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Quantified in Fig 3A.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS25: Sertoli cell |
Sertoli cell |
Present |
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Expressed in immature Sertoli cells. The number of Sertoli cells increases by 3.2-fold due to extensive proliferation.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * SJL
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Mutant Allele(s):
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Atrxtm1Rjg/Y
Plekha5Tg(AMH-cre)1Flor/0
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Age:
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embryonic day 17.5
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Sex:
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Male
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Conditional mutant. Quantified in Fig 3A.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS25: Sertoli cell |
Sertoli cell |
Present |
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No increase was measured, and Sertoli cell numbers were 28% of control numbers.
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Specimen
4A Control:
embryonic day 15.5; Atrxtm1Rjg/Y
(more )
Note: Double labeled: red - Amh; green - Casp3 (assay MGI:7751464).
Specimen
4A Control:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * SJL
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Mutant Allele(s):
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Atrxtm1Rjg/Y
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Age:
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embryonic day 15.5
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Sex:
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Male
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Type:
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section
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Embedding:
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Cryosection
|
|
Note:
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Double labeled: red - Amh; green - Casp3 (assay MGI:7751464).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: Sertoli cell |
Sertoli cell |
Present |
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4A left
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Expressed in immature Sertoli cells.
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Specimen
4A cKO:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * SJL
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Mutant Allele(s):
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Atrxtm1Rjg/Y
Plekha5Tg(AMH-cre)1Flor/0
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Age:
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embryonic day 15.5
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Sex:
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Male
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Type:
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section
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Embedding:
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Cryosection
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|
Note:
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Conditional mutant. Double labeled: red - Amh; green - Casp3 (assay MGI:7751464).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: Sertoli cell |
Sertoli cell |
Present |
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4A right
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Expressed in immature Sertoli cells.
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Specimen
5A Control:
embryonic day 16.5; Atrxtm1Rjg/Y
(more )
Note: Double labeled: red - Amh; green - PH3.
Specimen
5A Control:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * SJL
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Mutant Allele(s):
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Atrxtm1Rjg/Y
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Age:
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embryonic day 16.5
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Sex:
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Male
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Type:
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section
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Embedding:
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Cryosection
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|
Note:
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Double labeled: red - Amh; green - PH3.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: Sertoli cell |
Sertoli cell |
Present |
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5A left
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Expressed in immature Sertoli cells.
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Specimen
5A cKO:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * SJL
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Mutant Allele(s):
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Atrxtm1Rjg/Y
Plekha5Tg(AMH-cre)1Flor/0
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Age:
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embryonic day 16.5
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Sex:
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Male
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Conditional mutant. Double labeled: red - Amh; green - PH3.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: Sertoli cell |
Sertoli cell |
Present |
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5A right
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The number of PH3-positive Sertoli cells increased by 38%, compared with the control.
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Specimen
5B Control:
embryonic day 16.5; Atrxtm1Rjg/Y
(more )
Note: Double labeled: red - Amh; green - BrdU.
Specimen
5B Control:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * SJL
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Mutant Allele(s):
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Atrxtm1Rjg/Y
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Age:
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embryonic day 16.5
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Sex:
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Male
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Amh; green - BrdU.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: Sertoli cell |
Sertoli cell |
Present |
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5B left
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Expressed in immature Sertoli cells.
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Specimen
5B cKO:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * SJL
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Mutant Allele(s):
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Atrxtm1Rjg/Y
Plekha5Tg(AMH-cre)1Flor/0
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Age:
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embryonic day 16.5
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Sex:
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Male
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Conditional mutant. Double labeled: red - Amh; green - BrdU.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: Sertoli cell |
Sertoli cell |
Present |
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5B right
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The number of BrdU-positive Sertoli cells was reduced by 13% compared with the control.
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Specimen
S6 P7 Control:
postnatal day 7; Atrxtm1Rjg/Y
(more )
Specimen
S6 P7 Control:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * SJL
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Mutant Allele(s):
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Atrxtm1Rjg/Y
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Age:
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postnatal day 7
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Sex:
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Male
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: Sertoli cell |
Sertoli cell |
Present |
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S6_P7 upper left
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Expressed in immature Sertoli cells.
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Specimen
S6 P7 cKO:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * SJL
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Mutant Allele(s):
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Atrxtm1Rjg/Y
Plekha5Tg(AMH-cre)1Flor/0
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Age:
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postnatal day 7
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Sex:
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Male
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Conditional mutant.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: Sertoli cell |
Sertoli cell |
Present |
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S6_P7 upper right
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Expressed in immature Sertoli cells.
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Specimen
S6 P14 Control:
postnatal day 14; Atrxtm1Rjg/Y
(more )
Specimen
S6 P14 Control:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * SJL
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Mutant Allele(s):
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Atrxtm1Rjg/Y
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Age:
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postnatal day 14
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Sex:
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Male
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: Sertoli cell |
Sertoli cell |
Absent |
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S6_P14 upper left
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No expression in mature Sertoli cells.
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Specimen
S6 P14 cKO:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * SJL
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Mutant Allele(s):
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Atrxtm1Rjg/Y
Plekha5Tg(AMH-cre)1Flor/0
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Age:
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postnatal day 14
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Sex:
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Male
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Conditional mutant.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: Sertoli cell |
Sertoli cell |
Absent |
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S6_P14 upper right
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No expression in mature Sertoli cells, similar as in the control.
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