About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:74989 McEvilly RJ, et al., Transcriptional regulation of cortical neuron migration by POU domain factors. Science. 2002 Feb 22;295(5559):1528-32
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7751234
Gene symbol: Pou3f2
Gene name: POU domain, class 3, transcription factor 2
Antibody: anti-Brn-2 (2Gb)
Detection system: Alexa Fluor
Assay notes: Two Pou3f2 antibodies were described, but the one used in this assay was not detailed. Anti-Brn-2 (2R2) MGI:7751172 may have been used in this assay.
Results
Specimen 1A Brn-2 WT: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: cortical plate Present Regionally restricted 1A Brn-2 WT Expression in superficial cortical layers.
TS27: cerebral cortex ventricular zone Present 1A Brn-2 WT Expression in ventricular zone of neocortex.
Specimen 1A Brn-1/Brn-2 WT: postnatal day 0 (more )
Note: Double labeled: green - Pou3f2; red - Pou3f3 (assay MGI:7751233).
Structure Level Pattern Image Note
TS27: cortical plate Present Regionally restricted 1A Brn-1/Brn-2 WT Co-localization with Pou3f3 in numerous nuclei.
Specimen 1A Brn-2 Brn-1-/-: postnatal day 0; Pou3f3tm1Rmc/Pou3f3tm1Rmc (more )
Structure Level Pattern Image Note
TS27: cerebral cortex Present Regionally restricted 1A Brn-2 Brn-1-/- Distribution of expression unaffected compared to wild type.
Specimen 1A Brn-1/Brn-2 Brn-1-/-/Brn-2-/-: postnatal day 0; Pou3f2tm1Rsd/Pou3f2tm1Rsd, Pou3f3tm1Rmc/Pou3f3tm1Rmc (more )
Note: Double labeled: green - Pou3f2; red - Pou3f3 (assay MGI:7751233).
Structure Level Pattern Image Note
TS27: cerebral cortex Absent 1A Brn-1/Brn-2 Brn-1-/-/Brn-2-/-
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory