Antibody:	anti-Brn-1 (IG2)
Detection system:	Alexa Fluor

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cerebral cortex		Present	Regionally restricted	1A Brn-1 WT	Expression in superficial cortical layers.
TS27: cerebral cortex ventricular zone		Present		1A Brn-1 WT	Expression in ventricular zone of neocortex.
TS27: CA1 pyramidal cell layer	hippocampal pyramidal neuron	Present		1A Brn-1 WT	Expression in pyramidal neurons of CA1 field.

Structure	Level	Pattern	Image	Note
TS27: cortical plate	Present	Regionally restricted	1A Brn-1/Brn-2 WT	Co-localization with Pou3f2 in numerous nuclei.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Absent		1A Brn-1 Brn-1-/-

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Absent		1A Brn-1/Brn-2 Brn-1-/-/Brn-2-/-

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present	Regionally restricted	S3A e11-e19 Brn-1 , S3A e11-e19 Brn-1/BrdU	Virtually no colabeling with BrdU in neurons born at E11 and E12.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present	Regionally restricted	S3A e12-e19 Brn-1 , S3A e12-e19 Brn-1/BrdU	Virtually no colabeling with BrdU in neurons born at E11 and E12.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present	Regionally restricted	S3A e13-e19 Brn-1 , S3A e13-e19 Brn-1/BrdU	Expression in majority of neurons born on E13 and thereafter.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present	Regionally restricted	S3B e11-e16 (left) , S3B e11-e16 (right)	Virtually no colabeling with BrdU in neurons born at E11 and E12.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present	Regionally restricted	S3B e12-e16 (right) , S3B e12-e16 (left)	Virtually no colabeling with BrdU in neurons born at E11 and E12.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Present	Regionally restricted	S3B e13-e17 (left) , S3B e13-e17 (right upper) , S3B e13-e17 (right lower)	Expression in majority of neurons born on E13 and thereafter.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Present	Regionally restricted	S3B e14-e17 (right) , S3B e14-e17 (left)	Expression in majority of neurons born on E13 and thereafter.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Present	Regionally restricted	S3B e15-e17 (right) , S3B e15-e17 (left)	Expression in majority of neurons born on E13 and thereafter.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present	Regionally restricted	S3B e16-e18 (left) , S3B e16-e18 (right)	Expression in majority of neurons born on E13 and thereafter.

Structure	Level	Pattern	Image	Note
TS27: cortical layer V	Present	Regionally restricted	5C Tst-1/Brn-1	Expression colocalized with that of Pou3f1 in layer V neurons.

Structure	Level	Pattern	Image	Note
TS27: cortical plate	Present	Regionally restricted	5D Reelin/Brn-1	Expression in subpopulation of cortical plate neurons, co-localizing with that of Reln.