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Gene Expression Data
Assay Details
Assay
Reference: J:171452 Holtz ML, et al., Serum response factor is required for cell contact maintenance but dispensable for proliferation in visceral yolk sac endothelium. BMC Dev Biol. 2011;11:18
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7750935
Gene symbol: Srf
Gene name: serum response factor
Antibody: anti-SRF
Results
Specimen 1A: embryonic day 12.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sortm1(EYFP)Cos, Srftm1Rmn/Srftm1Rmn (more )
Note: Fixed in zinc fixative or zinc formalin. Double labeled: green - Srf; red - Cre recombinase reporter.
Structure Level Pattern Image Note
TS20: yolk sac mesenchyme Present 1A Expressed in the mesoderm.
Specimen 1B: embryonic day 12.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sortm1(EYFP)Cos, Srftm1Rmn/Srftm1Rmn, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Fixed in zinc fixative or zinc formalin. Double labeled: green - Srf; red - Cre recombinase reporter.
Structure Level Pattern Image Note
TS20: yolk sac mesenchyme Trace 1B Nearly absent.
Specimen 2A: embryonic day 12.5 (more )
Note: Fixed in zinc fixative or zinc formalin. Quantified in Fig 2C. Double labeled: green - Srf; red - Acta2 (assay MGI:7750937).
Structure Level Pattern Image Note
TS20: yolk sac mesenchyme Present 2A
Specimen 2B: embryonic day 12.5; Srftm1Rmn/Srftm1Rmn, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Fixed in zinc fixative or zinc formalin. Quantified in Fig 2C. Double labeled: green - Srf; red - Acta2 (assay MGI:7750937).
Structure Level Pattern Image Note
TS20: yolk sac mesenchyme Trace 2B Nearly absent (1 1%), compared that of wild-type (52 13%).
Specimen not shown: embryonic day 10.5 (more )
Note: Fixed in zinc fixative or zinc formalin. Quantified in Fig 2C.
Structure Level Pattern Image Note
TS17: yolk sac mesenchyme Present
Specimen not shown: embryonic day 10.5; Srftm1Rmn/Srftm1Rmn, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Fixed in zinc fixative or zinc formalin. Quantified in Fig 2C.
Structure Level Pattern Image Note
TS17: yolk sac mesenchyme Present Reduced expression (15 2%), compared that of wild-type (38 2%).
Specimen not shown: embryonic day 11.5 (more )
Note: Fixed in zinc fixative or zinc formalin. Quantified in Fig 2C.
Structure Level Pattern Image Note
TS19: yolk sac mesenchyme Present
Specimen not shown: embryonic day 11.5; Srftm1Rmn/Srftm1Rmn, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Fixed in zinc fixative or zinc formalin. Quantified in Fig 2C.
Structure Level Pattern Image Note
TS19: yolk sac mesenchyme Present Reduced expression (11 1%), compared that of wild-type (35 4%).
Specimen 4A: embryonic day 12.5 (more )
Note: Fixed in zinc fixative or zinc formalin. Double labeled: green - Srf; red - Vcl (assay MGI:7750939).
Structure Level Pattern Image Note
TS20: yolk sac mesenchyme Present 4A
Specimen 4B: embryonic day 12.5; Srftm1Rmn/Srftm1Rmn, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Fixed in zinc fixative or zinc formalin. Double labeled: green - Srf; red - Vcl (assay MGI:7750939).
Structure Level Pattern Image Note
TS20: yolk sac mesenchyme Trace 4B Nearly absent.
Specimen 7A: embryonic day 12.5 (more )
Note: Fixed in zinc fixative or zinc formalin. Double labeled: green - Srf; red - BrdU.
Structure Level Pattern Image Note
TS20: yolk sac mesenchyme Present 7A
Specimen 7C: embryonic day 12.5; Srftm1Rmn/Srftm1Rmn, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Fixed in zinc fixative or zinc formalin. Double labeled: green - Srf; red - BrdU.
Structure Level Pattern Image Note
TS20: yolk sac mesenchyme Trace 7C Nearly absent. But BrdU staining was increased compared with the wild-type (summarized in Fig 7E).
Specimen S2A: embryonic day 10.5 (more )
Note: Fixed in zinc fixative or zinc formalin. Double labeled: green - Srf; red - Acta2 (assay MGI:7750937).
Structure Level Pattern Image Note
TS17: yolk sac mesenchyme Present S2A
Specimen S2B: embryonic day 10.5; Srftm1Rmn/Srftm1Rmn, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Fixed in zinc fixative or zinc formalin. Double labeled: green - Srf; red - Acta2 (assay MGI:7750937).
Structure Level Pattern Image Note
TS17: yolk sac mesenchyme Weak S2B
Specimen S2C: embryonic day 11.5 (more )
Note: Fixed in zinc fixative or zinc formalin. Double labeled: green - Srf; red - Acta2 (assay MGI:7750937).
Structure Level Pattern Image Note
TS19: yolk sac mesenchyme Present S2C
Specimen S2D: embryonic day 11.5; Srftm1Rmn/Srftm1Rmn, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Fixed in zinc fixative or zinc formalin. Double labeled: green - Srf; red - Acta2 (assay MGI:7750937).
Structure Level Pattern Image Note
TS19: yolk sac mesenchyme Weak S2D
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03/25/2025
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