Dio3 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:218428 Martinez ME, et al., Genomic imprinting variations in the mouse type 3 deiodinase gene between tissues and brain regions. Mol Endocrinol. 2014 Nov;28(11):1875-86
Assay type:	RT-PCR
MGI Accession ID:	MGI:7750879
Gene symbol:	Dio3
Gene name:	deiodinase, iodothyronine type III

Probe:	Dio3-pF, Dio3-pR mutated allele
Assay notes:	Quantitative RT-PCR. Data represented as a percentage of expression from the paternal allele.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
Dio3^{Del m-/p+} Fetal body	E16.5	TS24: trunk	Present	1 µg; total RNA	Not Specified	Dio3^tm2Stg/Dio3⁺	Not Specified	Heterozygous mice that inherited the mutation from the mother (Dio3 m-/p+). Fetal body (excluding head and liver).
Dio3^{Del m+/p-} Fetal body	E16.5	TS24: trunk	Present	1 µg; total RNA	Not Specified	Dio3^tm2Stg/Dio3⁺	Not Specified	Heterozygous mice that inherited the mutation from the father (Dio3 m+/p-). Fetal body (excluding head and liver).
Dio3^{Del m-/p+} Fetal Liver	E16.5	TS24: liver	Present	1 µg; total RNA	Not Specified	Dio3^tm2Stg/Dio3⁺	Not Specified	Heterozygous mice that inherited the mutation from the mother (Dio3 m-/p+).
Dio3^{Del m+/p-} Fetal Liver	E16.5	TS24: liver	Present	1 µg; total RNA	Not Specified	Dio3^tm2Stg/Dio3⁺	Not Specified	Heterozygous mice that inherited the mutation from the father (Dio3 m+/p-).
Dio3^{Del m-/p+} Fetal Brain	E16.5	TS24: brain	Present	1 µg; total RNA	Not Specified	Dio3^tm2Stg/Dio3⁺	Not Specified	Heterozygous mice that inherited the mutation from the mother (Dio3 m-/p+).
Dio3^{Del m+/p-} Fetal Brain	E16.5	TS24: brain	Present	1 µg; total RNA	Not Specified	Dio3^tm2Stg/Dio3⁺	Not Specified	Heterozygous mice that inherited the mutation from the father (Dio3 m+/p-).
Dio3^{Del m-/p+} Adult Brain	P90	TS28: brain	Present	1 µg; total RNA	Not Specified	Dio3^tm2Stg/Dio3⁺	Not Specified	Heterozygous mice that inherited the mutation from the mother (Dio3 m-/p+).
Dio3^{Del m+/p-} Adult Brain	P90	TS28: brain	Present	1 µg; total RNA	Not Specified	Dio3^tm2Stg/Dio3⁺	Not Specified	Heterozygous mice that inherited the mutation from the father (Dio3 m+/p-).

Notes:
(a) Fetal tissue Dio3 mRNA levels were much lower in animals with paternal inheritance of the deletion (Dio3 Del m+/p-) than in heterozygous animals that inherited the deletion from the mother (Dio3 Del m-/p+). Dio3 mRNA levels in the adult brain were similar between alleles.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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