Antibody:	Nkx6.2 antibody
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS18: telencephalon	Weak

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Strong	Regionally restricted	5A	Strong expression confined to sulcus between lateral ganglionic eminence and medial ganglionic eminence.
TS20: lateral ganglionic eminence ventricular zone	Present	Scattered	5A	Expression in a few scattered cells in ventricular zone right next to domain of high expression.
TS20: medial ganglionic eminence ventricular zone	Present	Scattered	5A	Expression in a few scattered cells in ventricular zone right next to domain of high expression.

Structure	Level	Pattern	Image	Note
TS20: lateral ganglionic eminence ventricular zone	Present	Regionally restricted	5B	Ectopic expression within ventricular zone of ventral LGE.
TS20: medial ganglionic eminence	Present		5B	Expression expanded in MGE.

Structure	Level	Pattern	Image	Note
TS22: telencephalon ventricular layer	Present	Regionally restricted	5C , 5C inset	Expression in ventricular zone between lateral ganglionic eminence and medial ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS22: lateral ganglionic eminence	Strong	Regionally restricted	5D inset , 5D	Strong expression in domain that appears small, but is relatively larger considering that LGE itself is smaller in size compared to wild-type. Expression in scattered cells throughout much of LGE ventricular zone.
TS22: lateral ganglionic eminence ventricular zone	Present	Scattered	5D inset , 5D	Expression in scattered cells throughout much of LGE ventricular zone.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	6A	Expression in sulcus between lateral ganglionic eminence and medial ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS20: lateral ganglionic eminence ventricular zone	Present	Scattered	6B	Expression in scattered cells throughout much of LGE ventricular zone.

Structure	Level	Pattern	Image	Note
TS20: lateral ganglionic eminence	Present	Regionally restricted	6C	Ectopic expression in ventral part of LGE.

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present		6G

Structure	Level	Pattern	Image	Note
TS22: lateral ganglionic eminence ventricular zone	Present	Scattered	6H , 6H inset	Ectopic expression in scattered cells throughout most of LGE ventricular zone.

Structure	Level	Pattern	Image	Note
TS22: lateral ganglionic eminence	Strong	Widespread	6I	Expression significantly upregulated throughout LGE.