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Gene Expression Data
Assay Details
Assay
Reference: J:354661 He XD, et al., Overlapping peri-implantation phenotypes of ZNHIT1 and ZNHIT2 despite distinct functions during early mouse development. Biol Reprod. 2024 Aug 28;
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7736590
Gene symbol: Pou5f1
Gene name: POU domain, class 5, transcription factor 1
Antibody: anti-Oct4 (AF1759)
Detection system: Secondary antibody coupled to Alexa Fluor 647
Results
Specimen 3C Ctrl: embryonic day 4.0 (more )
Note: Triple labeled: green - Pou5f1; magenta - Cdx2 (assay MGI:7736572); blue - DAPI.
Structure Level Pattern Image Note
TS5: inner cell mass Present 3C Ctrl Merge , 3C Ctrl OCT4
Specimen 3C Mut: embryonic day 4.0; Znhit1em1(IMPC)J/Znhit1em1(IMPC)J (more )
Note: Triple labeled: green - Pou5f1; magenta - Cdx2 (assay MGI:7736572); blue - DAPI.
Structure Level Pattern Image Note
TS5: inner cell mass Present 3C Mut Merge , 3C Mut OCT4
Specimen 3D Ctrl: embryonic day 5.25 (more )
Note: Triple labeled: orange - Pou5f1; green - Cdx2 (assay MGI:7736572); blue - DAPI.
Structure Level Pattern Image Note
TS7: epiblast Present 3D Ctrl DAPI/OCT4/CDX2 , 3D Ctrl OCT4
Specimen 3D Mut: embryonic day 5.25; Znhit1em1(IMPC)J/Znhit1em1(IMPC)J (more )
Note: Mutant embryos were identified by phenotype. Triple labeled: orange - Pou5f1; green - Cdx2 (assay MGI:7736572); blue - DAPI.
Structure Level Pattern Image Note
TS7: epiblast Present 3D Mut OCT4 , 3D Mut DAPI/OCT4/CDX2
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory