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Gene Expression Data
Assay Details
Assay
Reference: J:354661 He XD, et al., Overlapping peri-implantation phenotypes of ZNHIT1 and ZNHIT2 despite distinct functions during early mouse development. Biol Reprod. 2024 Aug 28;
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7736572
Gene symbol: Cdx2
Gene name: caudal type homeobox 2
Antibody: anti-Cdx2 (CDX2-88)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 3C Ctrl: embryonic day 4.0 (more )
Note: Triple labeled: magenta - Cdx2; green - Pou5f1 (assay MGI:7736590); blue - DAPI.
Structure Level Pattern Image Note
TS5: trophectoderm Present 3C Ctrl CDX2 , 3C Ctrl Merge
Specimen 3C Mut: embryonic day 4.0; Znhit1em1(IMPC)J/Znhit1em1(IMPC)J (more )
Note: Triple labeled: magenta - Cdx2; green - Pou5f1 (assay MGI:7736590); blue - DAPI.
Structure Level Pattern Image Note
TS5: blastocyst Present 3C Mut Merge , 3C Mut CDX2 Although expression intensity is comparable to that seen in wild type, some expressing cells also retain Oct4 expression, suggesting incomplete trophectoderm differentiation.
TS5: trophectoderm Present 3C Mut Merge , 3C Mut CDX2 Although expression intensity is comparable to that seen in wild type, some expressing cells also retain Oct4 expression, suggesting incomplete trophectoderm differentiation.
Specimen 3D Ctrl: embryonic day 5.25 (more )
Note: Triple labeled: green - Cdx2; orange - Pou5f1 (assay MGI:7736590); blue - DAPI.
Structure Level Pattern Image Note
TS7: extraembryonic ectoderm Present 3D Ctrl CDX2 , 3D Ctrl DAPI/OCT4/CDX2
Specimen 3D Mut: embryonic day 5.25; Znhit1em1(IMPC)J/Znhit1em1(IMPC)J (more )
Note: Mutant embryos were identified by phenotype. Triple labeled: green - Cdx2; orange - Pou5f1 (assay MGI:7736590); blue - DAPI.
Structure Level Pattern Image Note
TS7: extraembryonic ectoderm Present 3D Mut CDX2 , 3D Mut DAPI/OCT4/CDX2
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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